山茱萸中一个新的环烯醚萜苷类化合物

A New Iridoid glucoside from the Ripe Fruits of Cornus officinalis Sieb. et Zucc.

目的研究山茱萸(Cornus officinalis Sieb.et Zucc.)成熟果实的化学成分及其对缺氧缺糖(oxygen-glucose deprivation,OGD)损伤PC12细胞的保护作用。方法采用大孔吸附树脂、硅胶、ODS、Sephadex LH-20柱色谱和制备HPLC等技术进行分离和纯化,结合理化性质及MS、NMR等波谱方法鉴定化合物的结构,并采用MTT法测定其对OGD损伤的PC12细胞的保护作用。结果从山茱萸成熟果实水提取物中分离得到8个化合物,分别为6'-O-乙酰基-7β-O-乙基莫诺苷(1),金圣草黄素(2),木犀草素(3),5-羟甲基糠醛(4),丁香酸(5),对羟基苯甲酸(6),咖啡酸甲酯(7)和没食子酸乙酯(8)。结论化合物1为新化合物,化合物2,3,5,7为首次从山茱萸植物中分离得到;化合物4在1.0μmol·L^(-1)的浓度下,对OGD/R损伤的PC12细胞具有一定的保护作用。

OBJECTIVE To study the chemical constituents from the ripe fruits of Comus officinalis Sieb. et Zucc. , and evaluate their protection on PC12 ceils exposed to oxygen and glucose deprivation. METHODS The compounds were isolated through various chromatographic methods including macroporous adsorption resin, silica gel, ODS, Sephadex LH -20 column chromatography, and preparative HPLC, and their structures were determined through spectroscopic analysis, including 1D and 2D NMR and MS da- ta. RESULTS Eight compounds were isolated from the water extract of the ripe fruits of Comus officinalis Sieb. et Zucc. , and their structures were elucidated as 6＇-O-acetyl-7β-O-ethyl morroniside （ 1 ）, chrysoderol （ 2）, luteolin （ 3 ）, 5-hydroxymethylfurfural （4）, sy- ringate（5） ,p-hydroxybenzoic acid（6） ,caffeic acid methyl ester（7）, ethyl gallate（8）. CONCLUSION Compound 1 is a new iridoid glucoside, and compounds 2, 3, 5, 7 are obtained from Comus officinalis for the first time. The MTF results show that compound 4 moderately increases the viability of OGD/R treated PC12 cells at the concentration of 1.0 μmol · L-1.