血根碱对宫颈癌细胞增殖、凋亡、侵袭、迁移能力影响机制研究

Study on effect mechanism of sanguinarine on proliferation,apoptosis,invasion and migration of cervical cancer cells

目的探讨血根碱对宫颈癌细胞增殖、凋亡、侵袭、迁移能力的影响机制。方法分别用MTT法、流式细胞仪、细胞划痕实验、Transwell小室检测血根碱作用后的宫颈癌细胞增殖、凋亡、迁移、侵袭能力。Western blot检测经血根碱作用后宫颈癌细胞中E-Cadherin、PTEN、β-catenin、MMP2的蛋白表达水平。结果 0.6、0.8μmol/L血根碱对宫颈癌细胞增殖具有抑制作用。0.8μmol/L血根碱作用48h后宫颈癌细胞HeLa和Siha凋亡率高达(45.68±2.26)%和(31.89±3.80)%。0.8μmol/L血根碱作用3h后宫颈癌细胞HeLa和Siha黏附率仅有(67.45±2.13)%和(73.59±2.61)%。0.8μmol/L血根碱作用16h后宫颈癌细胞HeLa和Siha侵袭数目仅有(39.64±1.98)个和(43.87±2.83)个。经血根碱作用后的宫颈癌细胞中E-Cadherin和PTEN的表达量增加,β-catenin和MMP2的表达量减弱。结论血根碱对宫颈癌细胞具有抑制增殖及促凋亡作用,其机制可能与黏附蛋白E-Cadherin、β-catenin和PTEN、MMP2有关。

Objective To investigate the effect mechanism of sanguinarine on the proliferation,apoptosis,invasion and migration abilities of cervical cancer cells. Methods MTT, flow cytometer, cell scratch test and Transwell chamber assay; were respectively used to detect the cellular proliferation,apoptosis,migration and invasion abilities after sanguinarine action. The expression levels of E-Cadherin, PTEN,β-catenin and MMP2 protein of cervical cancer cells after sanguinarine action were detected by Western blot. Results 0.6,0.8 9mol/L sanguinarine had the inhibitory effect on the proliferation of cervical cancer cells. After 0.8 μmol/L sanguinarine action for 48 h,cervical cancer HeLa and Siha cells apoptosis 1＂ate were up to （45.68±2.26）% and（31.89±3.80）% respectively. 0.8 μmol/L sanguinarine action for 3 h, cervical cancer cells H eLa and Siha adhesion rates were only （67.45±2.13） and（73.59±2.61）%. 0. 8 mol/L sanguinarine action for 16 h, the invasion numbers of cervical cancer H ela and Siha cell were （39.64±1.98） and （43.87±2.83） respectively. The expression amount of E-Cadherin and PTEN in cervical cancer cells after sanguinarine action was increased,while the expression amount of E-Cadherin and PTEN was weakened. Conclusion Sanguinarine has the proliferation inhibiting and apoptosis promoting effect on cervical cancer cells,its mechanism may be related to adhesion protein E-Cadherin,E-catenin and PTEN, MMP2.