元宝枫油对一次性力竭运动大鼠氧化应激和炎症反应的影响

Effects of Acer truncatum oil on oxidative stress and inflammatory reaction in rats undergoing one-time exhaustive exercise

目的探讨元宝枫油对一次性力竭运动大鼠氧化应激和炎症反应的影响,为元宝枫油用于预防运动损伤提供科学依据。方法 30只健康SD雄性大鼠根据体质量随机分为安静对照组(A组,正常饮食+每天生理盐水灌胃),力竭组(B组,正常饮食+每天生理盐水灌胃)和元宝枫油力竭组(C组,每天元宝枫油0.21mL/kg灌胃),6周后进行一次性力竭游泳运动,测定大鼠血清中肌酸激酶(CK)、乳酸脱氢酶(LDH)的活性及炎症因子白细胞介素(IL)-1β、IL-10和肿瘤坏死因子-α(TNF-α)的水平;测定肌肉匀浆中丙二醛(MDA)、还原性谷胱甘肽(GSH)水平及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)的活性。结果力竭运动后,B组与A组相比,血清中CK、LDH水平及IL-1β、TNF-α、IL-10水平均显著升高(P<0.05),IL-10/TNF-α比值差异无统计学意义(P>0.05),大鼠腓肠肌中MDA水平显著升高(P<0.05),GSH水平与GSH-PX活性显著下降(均P<0.05),SOD活性与CAT活性差异无统计学意义(P>0.05);C组与B组相比,血清中CK、LDH水平及IL-1β、TNF-α显著下降(均P<0.05),IL-10差异无统计学意义(P>0.05),IL-10/TNF-α比值显著上升(P<0.05),腓肠肌中MDA水平明显下降(P<0.05),GSH水平及SOD、CAT、GSH-PX活性均显著升高(均P<0.05)。结论元宝枫油可明显减轻力竭运动引起的氧化应激和炎症反应,对骨骼肌具有保护作用。

Objective To explore the effects of Acer truncatum oil on oxidative stress and inflammatory reaction in rats after exhaustive exercise to provide a scientific basis for the prevention of sports injury by Acer truncatum oil. Methods Thirty healthy male SD rats were randomly divided into control group （ A,normal diet ＋ saline every day）, exhaustive group （B,normal diet ＋ saline daily） and exhaustive with Acer truncatum oil group （ C,0.21 mL/kg daily Acer truncatum oil gavage）, 6 weeks later, the rats performed once exhaustive swimming exercise, then the creatine kinase （CK）,lactate dehydrogenase （LDH）, cytokine interleukin （IL-1β、 IL-10） and tumor necrosis factor alpha （TNF-α） in the serum;malondialdehyde （MDA） homogenate reduction glutathi- one （GSH）, superoxide dismutase （SOD）, catalase （CAT） and glutathione peroxidase （GSH-Px） in muscle were measured. Results After exhaustive exercise,when compared with the group A,the serum levels of CK,LDH, IL-1,TNF-α and IL-10 were significantly higher than in the group B（P〈0.05 ,P〈0.01 ,P〈0.05 ,P〈0.01 ,P〈0.05） ,IL-10/ TNF-α ratio had no statistically significant difference（P〈0.05）, the level of MDA in gastrocnemius muscle increased significantly （P〈0.05）, GSH level and GSH- PX activity decreased significantly （P〈0.05 ,P〈0.01） ,SOD and CAT activity were not statistically significant （P〈0.05）. when compared with the group B, serum CK, LDH level and IL-1β, TNF-α were significantly decreased （P〈0.05, P〈0.01, P〈 0.01, P〈0.05） ,IL-10 had no statistical significance （P〈0.05）, a significant increase in IL-10/TNF-^（P〈0.05）, the level of MDA of gastrocnemius muscle decreased significantly （P〈0. 05）, the level of GSH and SOD,CAT and GSH-PX were significantly increased （P〈0.01 ,P〈0.01,P〈0.05,P〈0.01）. Conclusion Aeer truncatum oil can significantly reduce oxidative stress and inflammatory reaction caused by the exhaustive exercise, which has a protective effect on skeletal muscle.