外周血Th17细胞表面CCR4、CCR6、CXCR3表达量与慢性乙型肝炎患者病情的相关性

Correlation of peripheral blood Th17 cell surface CCR4,CCR6 and CXCR3 expression with the illness in patients with chronic hepatitis b

目的:研究外周血Th17细胞表面CCR4、CCR6、CXCR3表达量与慢性乙型肝炎(CHB)患者病情的相关性。方法:选择在本院诊断为慢性乙型肝炎的48例患者作为研究的CHB组,同期体检的66例健康志愿者作为对照组,采集外周血并测定Th17细胞表面CCR4、CCR6、CXCR3的表达量,采集血清并测定Th17细胞因子、肝功能指标、肝纤维化指标的含量。结果:CHB组外周血Th17细胞表面CCR4、CCR6、CXCR3的荧光强度显著高于对照组;CHB组血清中IL-17、IL-21、IL-22、ALT、AST、GLB、HA、PC-III、LN、C-IV的含量显著高于对照组且与外周血Th17细胞表面CCR4、CCR6、CXCR3的荧光强度呈正相关,血清中ALB的含量显著低于对照组且与外周血Th17细胞表面CCR4、CCR6、CXCR3的荧光强度呈负相关。结论:CHB患者外周血Th17细胞表面CCR4、CCR6、CXCR3的高表达能够造成Th17细胞因子分泌增多、肝功能损害及肝纤维化。

Objective：To study the correlation of peripheral blood Th17 cell surface CCR4,CCR6 and CXCR3expression with the illness in patients with chronic hepatitis b（CHB）.Methods：A total of 48 patients who were diagnosed with chronic hepatitis b in Jianyang People＇s Hospital between July 2014 and January 2017 were selected as the CHB group of the research,66 healthy volunteers who received physical examination during the same period were selected as control group,the peripheral blood was collected to determine Th17 cell surface CCR4,CCR6 and CXCR3expression,and serum was collected to determine the levels of Th17 cytokines,liver function indexes and liver fibrosis indexes.Results：Peripheral blood Th17 cell surface CCR4,CCR6 and CXCR3fluorescence intensity of CHB group were significantly higher than those of control group;serum IL-17,IL-21,IL-22,ALT,AST,GLB,HA,PC-III,LN and C-IV levels of CHB group were significantly higher than those of control group and positively correlated with peripheral blood Th17 cell surface CCR4,CCR6 and CXCR3fluorescence intensity while serum ALB level was significantly lower than that of control group and negatively correlated with peripheral blood Th17 cell surface CCR4,CCR6 and CXCR3fluorescence intensity.Conclusions：The high expression of peripheral blood Th17 cell surface CCR4,CCR6 and CXCR3in patients with CHB can result in increased secretion of Th17 cytokines,liver function injury and liver fibrosis.