痤疮患者病灶及外周血中TLRs家族的表达量及其对炎症介质合成的调节作用

The TLRs family expression in lesions and peripheral blood of patients with acne and their regulating effect on the synthesis of inflammatory mediators

目的:研究痤疮患者病灶及外周血中Toll样受体(toll like receptors,TLRs)家族的表达量及其对炎症介质合成的调节作用。方法:选择2013年6月~2016年7月期间在我院就诊的60例寻常型痤疮患者作为痤疮组,同期在我院接受清创缝合的48例外伤患者作为对照组。收集痤疮病灶组织及清创后的皮肤组织并测定TLRs及炎症介质的表达量,采集外周血单个核细胞并测定TLRs的表达量。结果:痤疮组患者皮肤组织及外周血单个核细胞中TLR2、TLR4的蛋白表达量显著高于对照组(P<0.05),TLR3、TLR5、TLR7、TLR9的蛋白表达量与对照组比较差异无统计学意义(P>0.05);TLR2和TLR4高表达的痤疮组织中白介素(IL)-1α、IL-1β、IL-8、肿瘤坏死因子(TNF)-α的含量显著高于TLR2和TLR4低表达的痤疮组织(P<0.05)。结论:痤疮患者病灶及外周血中TLR2、TLR4呈高表达趋势且对炎症介质的合成具有促进作用。

Objective：To study the TLRs family expression in lesions and peripheral blood of patients with acne and their regulating effect on the synthesis of inflammatory mediators.Methods：60patients with acne vulgaris who were treated in our hospital between June 2013 and July 2016 were selected as the acne group,and 48 patients with trauma who accepted debridement and suturing in our hospital during the same period were selected as the control group.The acne focus tissue and the skin tissue after debridement were collected to determine the expression of TLRs and inflammatory mediators,and the peripheral blood mononuclear cells were collected to determine the expression of TLRs.Results：TLR2and TLR4 protein expression in skin tissue and peripheral blood mononuclear cells of acne group were significantly higher than those of control group while the TLR3,TLR5,TLR7 and TLR9protein expression were not significantly different from those of control group;IL-1α,IL-1β,IL-8and TNF-αlevels in acne tissue with high TLR2 and TLR4expression were significantly higher than those in acne tissue with low TLR2 and TLR4expression.Conclusion：TLR2and TLR4 are highly expressed in lesions and peripheral blood of patients with acne and have promoting effect on the synthesis of inflammatory mediators.