七种圈养鹤的部分血液生化指标分析

Comparison of Serum Biochemical Indices in Seven Captive Cranes Species(Gruiformes)

为探索圈养鹤类的血液生化指标差异,本研究对白鹤(Grus leucogeranus)、丹顶鹤(G.japonensis)、黑冠鹤(Balearica pavonina)、黑颈鹤(G.nigricollis)、灰冠鹤(B.regulorum)、蓝鹤(Anthropoides paradiseus)和白枕鹤(G.vipio)的10项血液生化指标(总胆固醇、甘油三酯、总蛋白、白蛋白、球蛋白等)进行统计分析,并与国际物种信息系统(ISIS)提供的数据进行比较。结果表明,1)不同鹤类之间总胆固醇差异显著,而甘油三酯和血糖没有显著性差异,除了黑冠鹤和灰冠鹤的总胆固醇以及蓝鹤的血糖外,其余鹤类的常值均高于ISIS的平均值。2)不同鹤类之间的总蛋白、球蛋白和白蛋白的浓度差异显著,但总蛋白、球蛋白和白蛋白均在ISIS的正常范围内。3)不同鹤类间肌酐和尿酸不具有显著性差异,其中尿酸低于ISIS平均值,但仅尿素氮差异显著。不同鹤类之间血液生化指标存在差异,与圈养鹤的营养水平和环境有关。因此血液生化指标对于圈养鹤类营养状态和健康情况的判断具有重要参考价值,对圈养动物饲养管理具有重要的指导作用。

For exploring the differences of serum biochemical indices for seven captive cranes species （Gruiformes）, including Siberian Crane （Grus leucogeranus）, Red-crowned Crane （G. japonensis）, Black-crowned Crane （Balearicapavonina）, Black-necked Crane （G. nigricollis）, Grey-crowned Crane （B. regulorum）, Blue Crane （Anthropoidesparadiseus） and White-naped Crane （G. vipio）, ten biochemical indicators including total cholesterol, triglyceride and serum glucose were measured in this study （Table 1）. Then, these measured values were compared with the average values provided by International Species Information System （ISIS） （Table 2）. All data were determined for assumption of normality by Shapiro-Wilk test and homogeneity of variance by Welch′s test. One sample T-test was used to compare with the average value of ISIS. LSD or Tamhane′s test was performed in different crane species, depending on the variances. The results indicated that, 1） there was significant difference on total cholesterol, however, no significant difference on triglyceride and serum glucose among these seven crane species. Except for total cholesterol of Black-crowned Crane, total cholesterol of Grey-crowned Crane and serum glucose of Blue Crane, other indicators are higher than the average value of ISIS （Table 2）. 2） There are significant differences in the concentration of total serum protein, albumin and globulins among these seven cranes species, with all these biochemical indicators in the range of ISIS （Table 3）. 3） There are no significant differences in the level of serum creatinine and uric acid, with the concentration of uric acid lower than the average value of ISIS, however, there is significant difference of the urea nitrogen among these seven crane species （Table 4）. The significant differences of serum biochemical indicators among crane species were mainly caused by feed and environmental factors. So the biochemical indicators of cranes provide an important reference for nutritional situation and healthy judgment, also a guiding role to breeding management for the captive animals.