3种三七样品中皂苷类成分的大鼠在体肠吸收特性比较

Comparison of in Situ Intestinal Absorption in Rats of Saponins in Three Kinds of Notoginseng Radix et Rhizoma Samples

目的:比较三七原枝粉、超微粉、破壁粉中三七皂苷R1,人参皂苷Rg_1,人参皂苷Rb_1的大鼠在体小肠吸收差异,为该药材的原料筛选提供依据。方法:采用大鼠在体循环灌流法,运用HPLC同时测定肠循环灌流液中三七皂苷R1,人参皂苷Rg_1,人参皂苷Rb_1及酚红的含量,研究3种三七样品中三七皂苷R1,人参皂苷Rg_1和人参皂苷Rb_1在大鼠小肠的吸收情况。结果:三七原枝粉、超微粉、破壁粉中三七皂苷R1的吸收速率常数(K_a)分别为0.049 80,0.037 37,0.034 60 h^(-1),人参皂苷Rg_1的K_a分别为0.044 83,0.032 48,0.036 50 h^(-1),人参皂苷Rb_1的K_a分别为0.078 40,0.095 48,0.084 78 h^(-1);三七皂苷R1的吸收率(P)分别为9.543%,7.662%,5.858%,人参皂苷Rg_1的P分别为8.602%,7.154%,6.667%,人参皂苷Rb_1的P分别为14.60%,17.86%,15.64%。对于三七皂苷R1的吸收,三七超微粉、破壁粉与原枝粉相比K_a和P均变小且存在显著性差异;对于人参皂苷Rg_1的吸收,三七超微粉、破壁粉与原枝粉相比K_a和P均变小;对于人参皂苷Rb_1的吸收,三七超微粉、破壁粉与原枝粉相比K_a和P均变大且超微粉存在显著性差异。结论:3种三七粉中三七皂苷R1,人参皂苷Rg_1和人参皂苷Rb_1在大鼠小肠的吸收量和吸收速率均存在一定差异,但三七超微粉和破壁粉的吸收情况并不比三七原枝粉好,建议原料选择三七原枝粉。

Objective：To compare the difference of in situ small intestinal absorption in rats of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in original powder,ultra-fine powder and cell wallbroken powder of Notoginseng Radix et Rhizoma.Method：In situ perfusion method in rats was adopted,the concentration of notoginsenoside R1,ginsenoside Rg1,ginsenoside Rb1 and phenol sulfonphthalein in intestinal circulating perfusate were determined simultaneously by HPLC,which is used to study on the absorption of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 from Notoginseng Radix et Rhizoma samples in the small intestine of rats.Result：The absorption rate constants（Ka） of notoginsenoside R1 in the original powder,ultrafine powder and cell wall-broken powder were 0.049 80,0.037 37,0.034 60 h-1,Kaof ginsenoside Rg1 were0.044 83,0.032 48,0.036 50 h-1,Kaof ginsenoside Rb1 were 0.078 40,0.095 48,0.084 78 h-1,respectively.The absorption percentages（P） of notoginsenoside R1 were 9.543%,7.662%,5.858%,those of ginsenoside Rg1 were 8.602%,7.154%,6.667%,those of ginsenoside Rb1 were 14.60%,17.86% and15.64%,respectively.Conclusion：There are differences in the absorption amount and rate of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in three kinds of Notoginseng Radix et Rhizoma powder,but the absorption of ultra-fine powder and cell wall-broken powder has no significant superiority by comparing with the original powder,selection of original powder as raw material is better.