复方梭子蟹胶囊对大鼠胃溃疡模型的影响

Effect of Compound Suozixie Capsule on Experimental Rat Models with Gastric Ulcer

目的:观察复方梭子蟹胶囊对4种大鼠胃溃疡模型的胃黏膜损伤的保护作用。并通过观察复方梭子蟹胶囊对慢性胃溃疡大鼠血清中胃组织中表皮生长因子(EGF),转化生长因子-α(TGF-α)和表皮生长因子受体(EGFR)蛋白表达的影响,初步探索其作用机制。方法:选取SD大鼠,采用水浸应激、消炎痛、幽门结扎、乙酸浸渍的方法构建4种大鼠胃溃疡模型,将大鼠随机分为正常组,模型组,复方梭子蟹胶囊低、中、高剂量组(2.07,4.14,8.28 g·kg^(-1)),阳性药组(法莫替丁7.2 mg·kg^(-1)),正常组和模型组分别灌胃纯水(15 mL·kg^(-1)),大鼠连续灌胃给药14 d。检测各组大鼠溃疡面积、抑制百分率。采用免疫组化法观察乙酸浸渍模型大鼠胃组织中EGF,TGF-α及EGFR蛋白表达的影响。结果:与正常组比较,模型组4种模型大鼠的溃疡面积显著升高(P<0.01);与模型组比较,复方梭子蟹胶囊能明显降低4种模型的溃疡面积(P<0.05,P<0.01),抑制溃疡损伤率,明显增加血清及胃组织中EGF,TGF-α和EGFR的蛋白表达(P<0.05,P<0.01)。结论:复方梭子蟹胶囊对大鼠水浸应激性胃溃疡、消炎痛型胃溃疡、幽门结扎型胃溃疡3种急性胃溃疡模型有一定的预防作用,对乙酸浸渍性慢性胃溃疡模型有治疗作用,并通过促进血清及胃组织中EGF,TGF-α和EGFR的蛋白表达来发挥抗慢性胃溃疡的作用。

Objective：To observe the protective effect of compound Suozixie capsule（CSC） on gastric mucosal injury in 4 kinds of experimental rat models with gastric ulcer,investigate the effects of CSC on levels of epidermal growth factor（EGF）,transforming growth factor-α（TGF-α） and epidermal growth factor receptor（EGFR） protein expression in serum and gastric tissues of rats with chronic gastric ulcer,and conduct preliminary exploration of its mechanism.Method：SD gastric ulcer model rats were induced by restraint water-immersion stress,indomethacin,pylorus ligating and acetic acid immersion respectively.Rats were divided into 5 groups randomly：normal control group,model control group,CSC low-dose group（2.07 g·kg-1）,medium-dose group（4.14 g·kg-1）,large-dose group（8.28 g·kg-1）,and positive control group（famotidine 7.2 mg·kg-1）.The rats in normal control group and model control group were given with pure water（15 mL·kg-1） by intragastric administration,and all the rats received intragastric administration of corresponding medicines for 14 days.Then the ulcer area and inhibition percentage were detected for all he rats in each group,and the protein expressions of EGF,TGF-α and EGFR in gastric tissues of acetic acid immersion model rats were determined by immunohistochemical staining.Result：As compared with the normal group,ulcer area was significantly increased in 4 kinds of model rats（P〈0.01）;as compared with the model group,CSC could significantly reduce the gastric ulcer area in four kinds of models（P〈0.05,P〈0.01）,inhibit the ulcer injury rate,and significantly increase the levels of EGF,TGF-α and EGFR protein expression in serum and gastric tissues（P〈0.05,P〈0.01）.Conclusion：The CSC had preventive effect on acute gastric ulcer models of stress-restrain,indomethacin,and pylorus ligating,and had therapeutic effect on chronic gastric ulcer model of acetic acid.Its anti-chronic gastric ulcer effect was associated with increasing the levels of EGF,TGF-α and EGFR protein expression in serum and gastric tissues.