身痛逐瘀汤对大鼠变性腰椎间盘内TNF-α、IL-1α、IL-6、MMP-3的影响

Effect of Shentong Zhuyu Decoction on tumor necrosis factor-α,interleukin-1α,interleukin-6 and matrix metalloproteinase-3 of rats' lumbar disc degeneration

目的观察身痛逐瘀汤对大鼠变性腰椎间盘内肿瘤坏死因子-α(TNF-α)、白介素-1α(IL-1α)、白介素-6(IL-6)、基质金属蛋白酶-3(MMP-3)水平的影响,研究中药调节炎性细胞因子分泌、延缓腰椎间盘变性的发生机制。方法本实验纳入8月龄雄性SD大鼠80只,按照随机分组法分为4组,20只SD大鼠作为正常对照组,60只建立腰椎间盘变性大鼠模型,随机分为空白对照组(BC)、西医治疗组(NASID)、中医实验组(ST)3组,每组各20只,西医治疗组选择双氯芬酸钠片连续灌胃给药,中医实验组以身痛逐瘀汤(泽兰合剂)进行灌胃给药,西医治疗组、空白对照组、正常对照组大鼠均给予等量生理盐水灌胃。通过分子生物学技术等观察相关指标,定性、定量分析TNF-α、IL-1α、IL-6、MMP-3水平。结果与空白对照组相比,西医治疗组和中医实验组治疗后TNF-α、IL-1α、IL-6、MMP-3水平均不同程度降低(P均<0.05);与正常对照组相比,TNF-α、IL-1α、IL-6、MMP-3水平有一定程度增高(P均<0.05);中医观察组与西医治疗组相比,TNF-α、IL-1α、IL-6、MMP-3下降更低(P<0.05)。结论身痛逐瘀汤与非甾体止痛抗炎药物均能减少大鼠变性腰椎间盘内TNF-α、IL-1α、IL-6、MMP-3的分泌,延缓腰椎间盘变性的进程,身痛逐瘀汤减少炎性因子分泌要好于非甾体类药物,不良反应也更少。

Objective We used SD rats to replicate human＇s lumbar disc degeneration （LDD） and evaluate the effect of Shentong Zhuyu Decoction on factor-α（TNF-α）, interleukin-1α（IL-1α）, interleukin-6（IL- 6） and matrix metallo proteinase-3 （MMP-3） of rats＇ lumbar disc degeneration （LDD）. Methods 8 month-old male SD rats were randomly di- vided into normal control group （NC group）, blank control group （BC group）, western medicine group （NASID group） and experimental group of Shentong Zhuyu Decoction （ST group）. According to the specific value converted the adult daily clinical administration, WC group were continued oral administration with diclofenac sodium for 30 days. The ST group were used Shentong Zhuyu Decoction （Zelan Mixture） to intragastric gavage （once a day） for 30 days. The BC group, NASID group and NC group were perfused with the same volume of saline for 30 days. The physiological saline dose was same with the ST group. After 30 days, all animals were killed and removed the rats lumbar disc tissue. TNF -α, IL-1α, IL- 6 and MMP-3 secretion level were analyzed with molecular biology method. Results TNF-α, IL -1α, IL-6 and MMP-3 levels of NASID group and ST group were lower than that of BC group （P〈0.05）. TNF-α, IL-1α, IL-6 andMMP-3 levels of ST group were lower than that of NASID group （P〈0.05）. Conclusion Shentong Zhuyu Decoction can reduce the expression of TNF-α, IL-1α, and IL-6 ,MMP-3 of rats lumbar disc and postpone the process of its degenera- tion.