紫色与非紫色芹菜花青素和芹菜素含量及合成基因表达分析

Analysis of Anthocyanin and Apigenin Contents and the Expression Profiles of Biosynthesis-related Genes in the Purple and Non-purple Varieties of Celery

高等植物中花青素合成与芹菜素合成之间存在共同的前体物质。利用非紫色芹菜‘六合黄心芹’和紫色芹菜‘南选六合紫芹’(从‘六合黄心芹’中选择而来)的叶柄为花青素和芹菜素代谢研究材料,利用荧光定量PCR方法检测花青素和芹菜素合成相关基因的表达水平。研究结果表明,‘六合黄心芹’叶柄中未检测到花青素积累,而‘南选六合紫芹’叶柄中的花青素含量呈现较高水平(0.0523 mg·g^(-1)FW)。‘六合黄心芹’叶柄芹菜素含量(0.0172 mg·g^(-1) FW)显著高于‘南选六合紫芹’(0.0124 mg·g^(-1) FW)。荧光定量PCR结果表明,除了AgFNS之外,其余花青素和芹菜素代谢相关基因(AgPAL、AgC4H、AgCHS、AgCHI、AgFNS、AgF3H、AgF3’H、AgDFR、AgANS和Ag3GT)在‘南选六合紫芹’叶柄中的表达量显著或者极显著高于‘六合黄心芹’;黄酮合成酶基因AgFNS在‘六合黄心芹’叶柄中的表达量为‘南选六合紫芹’的11.69倍。

In higher plant, biosynthesis of anthocyanin and apigenin share common precursor. In this study, petioles of non-purple celery, ＇Liuhe Huangxin Qin＇ and purple celery, ＇Nanxuan Liuhe Ziqin＇ （selected from ＇Liuhe Huangxin Qin＇） were used as materials for anthocyanin and apigenin biosynthesis research. The expression levels of anthocyanin and apigenin biosynthesis related genes （AgPAL, AgC4H, AgCHS, AgCH1, AgF3H, AgF3 ＇H, AgDFR, AgANS and Ag3GT genes）were also detected using qRT-PCR. The results showed that anthocyanins could not detected in the petioles of ＇Liuhe Huangxin Qin＇, and 0.0523 mg· g^-1 FW in ＇Nanxuan Liuhe Ziqin＇ . Amounts of 0.0172 mg· g^-1 FW apigenin in ＇Liuhe Huangxin Qin＇ and 0.0124 mg· g^-1 FW apigenin in ＇Nanxuan Liuhe Ziqin＇ were detected each per gram fresh materials, qRT-PCR results showed that except for AgFNS gene, the expression levels of AgPAL, AgC4H, AgCHS, AgCHI, AgF3H, AgF3 ＇H, AgDFR, AgANS and Ag3GTgenes were significant or very significant higher in the petioles of ＇Nanxuan Liuhe Ziqin＇ than that in the petioles of ＇Liuhe Huangxin Qin＇ The expression level ofAgFNS gene in the petioles of ＇Liuhe Huangxin Qin＇ was 11.69 times than that in the petioles of ＇Nanxuan Liuhe Ziqin＇