黄芪多糖免疫草鱼母本对子代IgM、C3和LSZ表达特性研究

The Expression Characteristics of IgM,C3 and LSZ in the Offspring from Female Ctenopharyngodon idella Immuned by Astragalus Polysaccharides

为探究黄芪多糖免疫草鱼母本所产子代早期发育阶段体液免疫因子IgM、C3和LSZ的表达特性及代间传递效率。采用ELISA、RT-qPCR等方法分析了饲喂黄芪多糖草鱼母本血液及其子代早期发育阶段3种免疫因子的蛋白质活性及mRNA水平。结果显示,黄芪多糖免疫草鱼母本血液中IgM、C3和LSZ蛋白活性均显著高于对照组。在子代早期阶段中,3种免疫因子蛋白活性呈先下降后上升趋势,实验组IgM蛋白活性在各阶段均显著高于对照组,在卵子、5d和28d仔鱼中分别提高了2.2倍、1.7倍和1.8倍。实验组C3活性在卵子、24h胚胎分别提高了1.9倍和1.6倍。实验组LSZ活性在卵子、5d和28d仔鱼中分别提高了2.4倍、2.0倍和1.9倍;在卵子和受精卵时期3种免疫因子mRNA水平显著高于对照组。在24h胚胎至5d仔鱼没有检测到IgM和LSZ mRNA,而在14d后3种免疫因子的mRNA均呈上调表达,但Ig M和C3 mRNA水平与对照组无显著差异。GCRV攻毒后,实验组2月龄草鱼脾脏和头肾中IgM mRNA水平显著高于对照组。结果表明,黄芪多糖能够提高草鱼母本免疫能力并向子代垂直传递,在应对GCRV感染时发挥一定免疫保护作用。

In order to explore the feature of Astragalus polysaccharides on the female parent, studied the common humoral immune factors about IgM, C3 and LSZ in fish, and discussed the expression of humoral immune factors （IgM, C3, LSZ） and the transfer effects of immunological factors in the early developmental stage of female offspring. The results showed that the activities of IgM, C3 and LSZ in the blood of the experimental group were significantly higher than those of the control group. In the early stage of the progeny, the activity of the three immune factors proteins showed a trend from decline to rise. The activity of IgM protein in the experimental group was significantly higher than that in the control group, and raised 2.2 times, 1.7 times, 1.8 times in the period of the egg stage, 5d and 28d. The activity of C3 in the experimental group was significantly different from that in the control group, raised 1.9 times and 1.6 times during the period of the egg stage, 24h. The offspring has no significant difference between the experiment group and the control group. The activity of LSZ was consistent with the activity of IgM protein, increased by 2.4 times, 2.0 times, 1.9 times during the egg stage, 5d, 28d. The mRNA expression of three immune factors in the period of the egg stage and zygote period was significantly higher than that in the control group, But the expression of IgM and C3 can＇ t find mRNAexpression during the period 24h -5d. However, the gene of three immune factors was up-regulated after 14d. After injecting with GCRV, the expression of IgM mRNA in the spleen and head kidney from the two-year- old grass carp was significantly higher than that of the control group. The results showed that the Astragalus polysaccharide could enhance the transformation of immune factors between generations, and play an important effect during the early development of filial generation.