硫化氢通过缺氧诱导因子1α促进张应力下骨髓间充质细胞的成骨

Hydrogen sulfide promotes the osteogenesis of bone marrow mesenchymal cells through hypoxia-inducible factor-1 alpha under tensile stress

背景:有研究表明,硫化氢信号能够促进牵张成骨,而具体机制尚不清楚。目的:观察硫化氢气体信号在促进张应力下大鼠骨髓间充质细胞成骨分化过程中与缺氧诱导因子1α的关系。方法:应用四点弯曲细胞加力装置系统,对体外分离培养的大鼠骨髓间充质细胞施加2 000μstrain的单一周期张应力,同时通过使用工具药物改变加力单元中硫化氢及缺氧诱导因子1α的含量水平,加力后检测成骨标志物的变化。结果与结论:(1)细胞成骨:外源性硫化氢信号能够促进张应力下大鼠骨髓间充质细胞成骨,而抑制加力单元缺氧诱导因子1α的表达则会减弱硫化氢信号的促进成骨作用;(2)结果说明:硫化氢信号系统可能通过缺氧诱导因子1α促进张应力下大鼠大鼠骨髓间充质细胞成骨作用。

BACKGROUND： Hydrogen sulfide signaling has been proved to promote distraction osteogenesis; however, the underlying mechanism remains unclear. OBJECTIVE： To evaluate the relationship between hydrogen sulfide and hypoxia-inducible factor-1α（HIF-1α）during the osteogenesis of rat bone marrow mesenchymal cells under tensile stress. METHODS： 2 000 μ strain was loaded on the in vitro cultured rat bone marrow mesenchymal cells by a four-point bending apparatus, and hydrogen sulfide donor or HIF-1α inhibitor was adopted in the tensile unit. Subsequently, the levels of osteogenic markers were detected. RESULTS AND CONCLUSION： Exogenous hydrogen sulfide signaling could promote the osteogenesis of rat bone marrow mesenchymal cells under tensile stress. However, this promotion was obviously eliminated when the endogenous HIF-1α expression was inhibited. These results show that hydrogen sulfide signaling system promotes the osteogenesis of rat bone marrow mesenchymal cells under tensile stress probably through HIF-1α.