垂序商陆及胜红蓟提取物对降香黄檀炭疽病菌的抑菌机理研究

Antifungal mechanisms of the ethyl acetate extracts from Phytolacca americana and Ageratum conyzoides against Colletotrichum gloeosporioides of Dalbergia odorifera

通过测定垂序商陆与胜红蓟乙酸乙酯提取物处理后的降香黄檀炭疽病菌内丙二醛含量、蛋白质含量以及4种酶活性,来初步判断垂序商陆与胜红蓟提取物对炭疽病菌的作用机理。结果表明:两种提取物都能在一定程度上破坏炭疽病病原菌膜系统,垂序商陆处理组丙二醛含量在56h后显著提高,胜红蓟处理组则缓慢增加,略高于对照组;垂序商陆处理组菌体内蛋白质含量先增加,32h后急剧降低,胜红蓟处理组蛋白质含量从初期开始就少于对照组;两种提取物都能影响菌体的保护性酶活性,两处理组SOD活力都呈现先增高后降低的趋势,CAT活力高于对照组。两组SDH、LDH活力都小于对照组,而垂序商陆处理组SDH、LDH活力小于胜红蓟处理组。初步说明两种植物提取物可通过影响病原菌的代谢系统和生理过程来发挥其抑菌作用。

To judge the action mechanism of ethyl acetate extracts fromPhytolacca americana and Ageratum conyzoides against Colletotrichum gloeosporioides,the MAD content,protein content and the activity of 4 kinds of enzymes of the pathogen treated with the extracts were measured.The results showed that the membrane system of the pathogen could be partly destroyed by the extracts of 2 plants;the MDA content of the pathogen treated with the extract of P.americanaincreased significantly 56 h later,and the MDA content of the pathogen treated with the extract of A.conyzoides increased slowly,slightly higher than the control group.The protein content of the pathogen treated with the extract of P.americanaincreased32 h after treatment,and then decreased rapidly.The protein content of the pathogen treated with the extract of A.conyzoides was less than that of the control group from the beginning on.The activity of protective enzymes was affected by the extracts of both plants.The SOD activity tended to increase firstly and decrease subsequently,and the CAT activity of the two groups was higher than that of their control group.The SDH and LDH activity was inhibited by the extracts of both plants,while the inhibitory effect of P.americana extracts was stronger than that of A.conyzoides extracts.In addition,it was found that the two extracts could destroy the metabolic pathways and affect the physiological function of the pathogen,which might be the reason why the extracts could inhibit C.gloeosporioides.