摘要
小鼠上胚层干细胞(mouse epiblast stem cell,mEpiSC)是一种来自于5.5天~7.5天小鼠胚胎上胚层(epiblast)组织的多能性干细胞。EpiSC自我更新能力和多能性的维持主要依赖激活素(Activin/Nodal)和成纤维细胞生长因子(fibroblast growth factor,FGF)信号,Activin和FGF信号对细胞多能网络进行调控,EpiSC培养体系和分离方法目前仍处于不断优化的过程。相比于处于"幼稚(na?ve)"状态的小鼠胚胎干细胞(mouse embryonic stem cell,mESC),mEpiSC被认为处于"待发(primed)"状态。虽然mEpiSC被注射入囊胚后,细胞嵌合效率较低,但是当其被移植入着床后胚胎时,却比mESC更容易与受体胚胎发生嵌合。因此,来源于小鼠着床后胚胎的EpiSC与来自于着床前胚胎的ESC相比,在多能性维持、发育潜能和诱导方法等方面存在本质区别。该文对小鼠EpiSC和ESC进行比较,并综述目前小鼠EpiSC的研究进展。
Mouse epiblast stem cell(mEpiSC) is derived from the epiblast tissue of mouse 5.5 days-7.5 days embryos. Self-renewal and pluripotency of EpiSC is maintained by Activin/Nodal and FGF(fibroblast growth factor). Activin and FGF regulate pluripotency network. Culture system of EpiSC and separation methods still being explored. Compared with "na?ve" state of mouse embryonic stem cell(mESC), mEpiSC is thought to be in a state of "primed". When mEpiSC is injected into blastocysts, the efficiency of chimeras is low. But it was easier to fuse with the implantation embryo when compared with mESC. Therefore, the EpiSC derived from the post-implantation embryo had essential difference compared with the mESC derived from the pre-implantation embryo, such as maintenance of pluripotent, developmental potential and induction method. In this review, EpiSC and ESC of mouse were compared, and a brief summary of recent advances in the knowledge of EpiSC had been given.
出处
《中国细胞生物学学报》
CAS
CSCD
2017年第7期931-938,共8页
Chinese Journal of Cell Biology
基金
内蒙古大学提升综合实力项目(批准号:11200-12110201)资助的课题~~