烟草特有亚硝胺分子印迹微球的制备及应用

Preparation and application of molecularly imprinted polymers for selective adsorption of tobacco specific nitrosamines

为研发出能够有效吸附烟草特有亚硝胺(TSNAs)的新型材料,以烟酰胺为模板、甲基丙烯酸为功能单体、二乙烯基苯为交联剂,采用沉淀聚合法制备了以吸附TSNAs为目标的分子印迹聚合物(MIPs)微球,并进行了MIPs结构表征、吸附性能评价及离线固相萃取应用。结果表明:采用本方法制备的MIPs具有较高的比表面积和吸附容量,在4-(N-甲基N-亚硝氨基)-1-(3-吡啶基)-1-丁酮(NNK)溶液中30min内就可达到吸附平衡,吸附率达到96%;将MIPs作为填料制备离线固相萃取小柱时,可实现烟草萃取液中TSNAs在MIPs固相萃取柱上的富集,吸附效率接近100%,且具有良好的可重复性;4种TSNAs在MIPs固相萃取柱上的加标回收率范围是66%~113%;与商品化的在线固相萃取色谱柱进行对比,MIPs离线固相萃取柱对TSNAs的回收率和响应强度与在线固相萃取色谱柱接近,有较好的应用前景。

For selectively adsorbing tobacco-specific nitrosamines（TSNAs）, molecularly imprinted polymers（MIPs） were prepared by precipitation polymerization with nicotinamide, methacrylic acid and divinyl benzene as reagents.The MIP structures were characterized and their adsorption performance was assessed.The results showed that the prepared MIPs with higher specific surface areas in NNK solutions possessed excellent adsorption efficiency（96%） and short adsorption time（30 min） for the target molecules.Furthermore, MIPs were used in an offline solid-phase extraction to selective extraction of TSNAs in tobacco leaves and the adsorption efficiency was about 100%, and the MIPs column could be reused.The standard recoveries of MIPs column for TSNAs ranged from 66% to 113%.The MIPs column was compared with online solid-phase extraction column to exhibit its potential application with the close recovery and intensity for online solid-phase extraction of TSNAs.