葛花对缓解小鼠急性酒精中毒的影响

Alleviation effect of Puerariae flos on acute alcoholism in mice

为了探讨葛花对急性酒精中毒的缓解作用及机理,根据中医烟熏疗法的作用方式向烟丝中添加一定量的葛花,分别采用酒精+主流烟气暴露(处理1)和主流烟气暴露+酒精(处理2)的方式处理小鼠,考察了实验小鼠的醒酒效果和血液中乙醇浓度及肝脏中乙醇脱氢酶活性的变化。结果表明:(1)与空白组相比,处理1在高剂量葛花添加组能显著缩短醉酒时间,处理2随着葛花添加量的增加能显著延长酒精耐受时间及缩短醉酒时间;(2)葛花添加量的增加可使处理2小鼠肝脏中乙醇脱氢酶活性升高,血液中乙醇浓度随时间呈现明显下降趋势。烟丝中添加葛花预处理对小鼠急性酒精中毒具有较为明显的缓解作用。

In order to investigate the alleviation effect and mechanism of Puerariae flos on acute alcoholism, a test cigarette was prepared by adding a certain amount of Puerariae flos into cut tobacco.According to the fumigation therapy theory and action mode of traditional Chinese medicines, experimental mice were treated by treatment 1（gastric lavage with alcohol ＋ mainstream cigarette smoke exposure） and treatment 2（mainstream cigarette smoke exposure ＋ gastric lavage with alcohol） separately, then the antialcoholism effects and the variations of alcohol concentration in blood and alcohol dehydrogenase activity in liver of mice were investigated.The results showed that： 1） Comparing with control groups, the drunkenness time of treatment 1group significantly reduced when a high dosage of Puerariae flos was added into cigarette; for treatment 2group, alcohol tolerance time remarkably increased, while drunkenness time decreased with the increase of Puerariae flos adding rate.2） Increasing Puerariae flos adding rate raised the alcohol dehydrogenase activity in liver, while decreased the alcohol concentration in blood obviously along with the proceeding of time.Mainstream smoke of cigarette pretreated by adding Puerariae flos into cut tobacco has an obvious alleviation effect on acute alcoholism in mice.