不同营养状况儿童白细胞端粒长度与血压的相关性分析

Association between telomere length and blood pressure among different nutritional status children

目的分析儿童端粒长度与血压水平以及血压偏高检出率之间的关联性,为有效预防儿童高血压提供参考。方法在2012年卫生行业科研专项基线数据库中,按照体质量指数(BMI)筛选湖南、天津、辽宁、上海4省市7~12岁儿童253名,按照BMI水平分为正常体重(81名)、超重和轻中度肥胖(85名)、重度肥胖(87名)3组,采用实时荧光定量PCR方法测定白细胞端粒长度,并计算对数转换的端粒长度。采用Pearson相关分析端粒长度与血压水平之间的关联性。结果采用美国国家高血压教育项目(NHBPEP)标准和中国高血压联盟(CHL)标准,253名7~12岁儿童的血压偏高检出率分别为15.81%和24.90%,男生分别为17.36%和28.93%,女生分别为14.39%和21.21%,男生均高于女生,差异均无统计学意义(P值均>0.05)。基于NHBPEP标准,血压偏高组儿童的端粒检测长度(TLT)和对数转换长度(Log-TLT)分别为1.07和0.02,与正常血压组儿童长度(分别为1.09和0.03)差异均无统计学意义(P值均>0.05);基于CHL标准,血压偏高组儿童的端粒长度TLT和Log-TLT分别为1.09和0.03,与正常血压组儿童长度(1.08和0.03)差异均无统计学意义(P值均>0.05)。男、女生呈现相同趋势,差异无统计学意义(P>0.05)。在BMI正常、超重和轻中度肥胖、重度肥胖3组学生中基本呈现相同趋势。结论儿童时期端粒长度与血压水平和血压偏高检出无明显的关联性,还需要进一步在成人人群中进行验证。

Objective This study aims to determine the association between the average leukocyte telomere length（ LTL）and the level of blood pressure（ BP） in children and to provide basis for the effective prevention of hypertension of children. Methods From the special health research baseline database,a total of 253 children aged 7 to 12 years were selected from Hunan, Tianjin, Liaoning and Shanghai in 2012 based on body mass index（ BMI）. Participants were categorized into three groups including normal BMI, overweight/moderate obese and severe obese group, with 81, 85 and 87 children in each group. Multiplex quantitative real-time PCR was used to measure leukocyte telomere length and calculate the log-transformed length. The association between LTL and the level of BP was analyzed by using Pearson correlation analysis. Results The elevated BP in children aged 7-12 were 15.81% and 24.90% according to National High Blood Pressure Education Program（ NHBPEP） and Chinese Hypertension League（ CHL）, respectively. The prevalence was 17.36% and 28.93% in boys, and 14.39% and 21.21% in girls, boys was higher than that of girls（ P〉0.05）. The LTL and log-LTL was 1.07 and 0.02 in elevated BP（ NHBPEP） group and 1.09 and 0.03 in elevated BP（ CHL） group, no differences were found between those two groups with normal blood pressure group（ P〈0.05）. Boys and girls showed the same results. The LTL and log-LTL showed no significant differences among normal BMI, overweight/moderate obese and severe obese group. Conclusion There are no significant association between telomere length with normal and elevated blood pressure. However, the results should be validated further in a large population of adults.