极低频电磁场对小鼠血清趋化因子水平的影响

Effects of extremely low frequency electromagnetic field on levels of chemokines in mouse serum

目的探讨极低频电磁场对小鼠血清趋化因子水平的影响。方法将130只健康4~6周龄SPF级雄性BALB/c小鼠随机分为5组,分别为空白(第0天)组(10只)及0(对照)、0.1、0.5、2.5 mT辐照组(各30只)。采用50 Hz极低频电磁场辐照小鼠,每天照射8 h;分别于第1、10、30天,测定小鼠血清中趋化因子单核细胞趋化蛋白-1(MCP-1)、嗜酸性粒细胞趋化因子(EOTAXIN-1)、MCP-3、巨噬细胞炎症蛋白-1α(MIP-1α)、MIP-1β、MIP-2、γ干扰素诱导的蛋白-10(IP-10)、生长相关癌基因-α(GRO-α)、正常T细胞表达分泌的活性调节蛋白(RANTES)的水平。结果与对照组相比,0.5 mT辐照第30天时小鼠血清MCP-1、GRO-α的水平及0.5、2.5 mT辐照第30天时小鼠血清MIP-1β水平以及各剂量辐照第10天、0.5 mT辐照第20天和0.1、0.5 mT辐照第30天时小鼠血清EOTAXIN-1的水平均较高,而0.5 mT辐照第10天时小鼠血清IP-10的水平和0.1 mT辐照第30天时小鼠血清RANTES的水平均较低,差异均有统计学意义(P<0.05);各剂量辐照不同时间小鼠血清MCP-3、MIP-1α、MIP-2的水平均无明显改变。结论极低频电磁场对小鼠血清趋化因子MCP-1和EOTAXIN-1有刺激作用,EOTAXIN-1有可能作为一种稳定的生物标志物用于研究极低频电磁场对人体的影响。

Objective To study the effects of extremely low frequency electromagnetic field on the levels of chemokines in mouse serum. Methods One hundred and thirty BALB/c male mice of SPF grade were randomly divided into five groups：control（day 0） group（10 mice）,control（0 mT） group（30 mice）, 0.1 mT group（30 mice）, 0.5 mT group（30 mice） and 2.5 mT group（30 mice）. The mice were exposed to 50 Hz extreme low frequency electromagnetic field for eight hours one day. The levels of monocyte chemoattractant protein 1（MCP-1）, EOTAXIN-1, MCP-3, macrophage inflammatory protein 1α（MIP-1α）,MIP-1β, MIP-2, interferon-γ inducible protein-10（IP-10）, growth-related oncogene-α（GRO-α） and RANTES in serum were tested after exposure for 1, 10 and 30 days. Results The concentrations of MCP-1, GRO-α of 0.5 mT group and MIP-1β of0.5 mT group, 2.5 mT group after exposure for 30 days were higher than that of the control group（P0.05）. The concentrations of EOTAXIN-1 significantly increased after exposure for 10 days（P0.05）. The concentrations of EOTAXIN-1 of 0.5 mT group after exposure for 20 days and 0.1 mT group, 0.5 mT group after exposure for 30 days were also observed compared with control group（P0.05）. The concentrations of IP-10 of 0.5 mT group after exposure for 10 days and RANTES of 0.1 mT group after exposure for 30 days significantly decreased（P 0.05）. No significant differences in the levels of MCP-3, MIP-1α and MIP-2 between control group and exposure groups were observed（P0.05）. Conclusion The levels of MCP-1 and EOTAXIN-1 in mouse serum can be stimulated by the exposure of extremely low frequency electromagnetic field. EOTAXIN-1 may serve as a stabilized biomarker to detect exposure to extreme low frequency electromagnetic field.