农达对小鼠生精作用的影响

Effects of Roundup on spermatogenic function on male mice

目的探讨农达(roundup,活性成分为草甘膦)对小鼠生精作用的影响。方法将24只成年SPF级雄性Balb/c小鼠随机分为阴性对照(蒸馏水)组和20、200、2 000 mg/kg农达染毒组,每组6只。采用经口灌胃方式进行染毒,染毒容量为20 ml/kg,每天1次,连续染毒30 d。测定小鼠精子数量、精子活动率和血清中睾酮(T)浓度及睾丸生精细胞的凋亡情况。结果与阴性对照组相比,200、2 000 mg/kg农达染毒组小鼠精子数量减少、精子活动度降低;各剂量农达染毒组小鼠血清睾酮浓度均降低,而睾丸生精细胞的凋亡率均升高,差异均有统计学意义(P<0.05)。结论在本实验染毒时间和剂量范围内,农达对雄性Balb/c小鼠的生精功能有一定的毒性作用。

Objective To observe the effect of Roundup（active ingredient is glyphosate） on spermatogenic function of mice.Methods A total of 24 SPF male Balb/c mice were randomly divided into three experimental groups and one control group. Six mice in each group were treated with solution of roundup（20 ml/kg）through gavage at the dose of 0,20,200,2 000 mg/kg body weight once a day for 30 consecutive days.The sperm count and sperm motility in epididymides,testosterone（T） levels of serum and the apoptosis of spermatogenic cells were measured among miceon day 31. Results Compared with the control group,the number and motility of sperm were decreased in 200 and 2 000 mg/kg treated groups（P0.05）. Testosterone levels of serum in each group were decreased significantly,however,apoptosis rate of spermatogenic cell were increased significantly（P 0.05）Conclution Herbicide Roundup may effect the function of spermatogenesis on male Balb/c mice within the range of period and dose.