一种新型罗丹明类双通道荧光探针及其对Fe^(3+)和Cu^(2+)的选择性识别研究

Synthesis of New Dual Fluorescent Probe Based on Rhodamineand Performance in Selective Recognition of Fe^(3+) and Cu^(2+)

以罗丹明B、水合肼和吡啶-2-甲醛为反应物,合成了一种新型的罗丹明类Fe^(3+)和Cu^(2+)双通道分子探针(Fluorescent probe,FP),并用核磁和质谱对其分子结构进行了表征。利用荧光分光光度计和紫外可见分光光度计研究了FP对Fe^(3+)、Cu^(2+)等14种金属离子的识别性能。研究结果表明,在V(CH_3OH)∶V(H_2O)=1∶1体系中,当选用荧光光谱分析时,探针FP对Fe^(3+)具有良好的选择性响应,且Fe^(3+)浓度在2~30μmol/L时,探针荧光强度与Fe^(3+)浓度呈线性关系,线性相关系数为0.998。当选用紫外可见光谱分析时,探针FP对Cu^(2+)具有很好的选择性响应,且当Cu^(2+)浓度为0~9μmol/L时,探针FP的吸光度与Cu^(2+)浓度呈线性关系,线性相关系数为0.999。

A novel Rhodamine-based dual fluorescent probe （FP） for detecting Fe3＋ and Cu2＋ was designed and synthesized using Rhodamine B,hydrazine hydrate and pyridine-formaldehyde as raw reactants. The molecule structure of FP was confirmed by 1HNMR and MS, and the selective recognition properties in detecting Fe3＋ , Cu2＋ and other 12 different kinds of metal ions were investigated by fluorescent spectroscopy and UV-Vis spectroscopy.The results revealed that FP had a high selectivity and sensitivi- ty for Fe3＋ using fluorescence spectra, and for Cu2. via UV-Vis absorption spectra in V（CH3OH） ：V（H2O） = l：l.When the con- centration of Fe3＋ and Cu2＋ was respectively in 2-30 μmol/L and 0~ 9 μmol/L,there was a good linearity relationship between the targeted metal ion of Fe3＋ and Cu2＋ and their fluorescence intensity and UV-Vis absorption, and the coefficient values were more than 0. 998.