D-核糖对小鼠抗疲劳作用的影响

Antifatigue Effect of D-ribose on Mice

目的:研究D-核糖对小鼠力竭游泳时间、肝糖元及血清尿素的影响,探讨D-核糖抗疲劳作用的机制,为核糖作为运动营养补充剂提供依据。方法:利用随机分组法将180只昆明种小鼠分为3批,每批6组,即5个实验剂量组和1个空白对照组,每组10只;连续30d经口给予D-核糖,末次灌胃后进行负重力竭游泳实验、肝糖原的测定以及血清尿素的测定;实验动物饲喂期间每周进行称重。结果:D-核糖对小鼠体重的增长具有明显的控制作用(P<0.05、P<0.01),能够显著延长小鼠负重游泳时间,时间延长率最高可达91.46%;实验组动物的血清尿素水平与对照组相比存在显著性差异(P<0.05);最低剂量组(125mg/kg·BW)能显著提升肝糖原含量(P<0.05)。结论:D-核糖能够明显延长小鼠力竭游泳时间,增强肝糖元的储备能力,降低血清尿素的产生,对延缓疲劳和体重增长的控制具有积极作用。

[Objective] To discuss the effect of D-ribose on anti-fatigue and the mechanism of anti-fatigue effect on mice , which can provide a basis for the ribose as sports nutrition supplements. [Method] Totally 180 mice were divided into 3 groups randomly, each consis- ting of six groups, which have five experimental groups and a control group ; the mice were exposed via the diet to D- ribose, for 4 consecu- tive weeks. To discuss the effect of D-ribose on anti-fatigue through the weight load exhausting swimming test and the analysis of serum urea changes and hepatic glycogen. [Result] D- ribose could significantly prolong swimming time, the highest prolonged rate is 91. 46% ; and there was a significant reduction in serum urea generation. The lowest dose group could significantly improve glycogen content （ P 〈 0. 05 ）, but the remaining four experimental groups of mice had no significant effect on the hepatic glycogen. [Conclusion] D- ribose can significantly improve sports endurance in mice and enhance the ability of anti-fatigue.