一测多评法测定玄参中7种有效成分的含量

A quantitative method for simultaneous assay of seven active ingredients with one marker in Scrophularia ningpoensis root

以玄参为研究对象,探索同步测定玄参中环烯醚萜类和苯丙素类共7种有效成分的高效液相色谱条件,并在此基础上建立"一测多评"测定方法,验证其准确性。以梓醇为内标物,建立梓醇与桃叶珊瑚苷、哈巴苷、毛蕊花糖苷、安格洛苷C、哈巴俄苷、肉桂酸的相对校正因子与相对保留值,计算其他6种成分的含量:同时采用外标法测定这7种成分的含量,比较两者的测定结果。结果表明采用"一测多评"法和外标法测定的含量之间无显著差异,实验所得的校正因子可信。该方法作为一种新的质量评价模式,用于玄参多种有效成分的同步质量评价是准确、可行的。

To establish a new quantitative method for simultaneous determination of multi-components in Scrophularia root by using high performance liquid chromatography （HPLC） and validate its feasibilities. Meanwhile, using catalpol as one chemical reference sub- stance to establish the relative correct factors and relative retention values of aucubin, harpagide, acteoside, angoroside C, harpagoside and cinnamic acid. Then using the quantitative analysis of multi-components by single-marker （QAMS） model, the six analytes can be quantitatively determined in Scrophularia root. The method was evaluated by comparison of the quantitative results to external standard method. No significant differences were observed between the quantitative results of the two methods. The obtained RCFs were credible. It is feasible and suitable to evaluate the quality of Scrophularia root.