中风病痰湿体质患者载脂蛋白E基因多态性分布特点研究

Characteristics of ApoE Gene Polymorphism Distribution in Stroke Patients of Phlegm-dampness Constitution

目的探讨中风病痰湿体质与载脂蛋白E(ApoE)基因多态性之间的相关性。方法纳入中风病痰湿体质患者77例(观察组),中风病非痰湿体质患者80例(对照组)。收集患者的一般资料,检测血脂及血清载脂蛋白水平,包括甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、载脂蛋白A1(ApoA1)、载脂蛋白B(ApoB)、脂蛋白(a)[Lp(a)]、ApoE水平,并检测ApoE基因多态性。结果与对照组比较,观察组血清中TC、TG、LDL-C、ApoE、ApoB水平均升高,HDL-C水平及ApoA1/ApoB降低(P<0.05或P<0.01),而Lp(a)、ApoA1水平比较差异均无统计学意义(P>0.05)。共检出3种基因型( ε3/ ε3、ε2/ ε3、ε3/ ε4)和3种等位基因( ε2、ε3、ε4)。两组基因型及等位基因分布经哈迪-温伯格平衡检验比较示差异无统计学意义(P>0.05),符合哈迪-温伯格遗传平衡。两组ApoE基因型及其等位基因整体分布情况差异均有统计学意义(P<0.05)。与对照组比较,观察组携带ApoE ε4等位基因及ApoE ε3/ ε4基因型患者较多(P<0.05),其余基因型及等位基因在两组间的分布差异均无统计学意义(P>0.05)。结论中风病痰湿体质易患中风可能与携带ApoE ε4等位基因、ApoE ε3/ ε4基因型有关,痰湿体质者血脂浓度较高可能受ApoE基因多态性调控。

Objective To explore the correlation between the phlegm-dampness constitution and the apohpopro- tein E （ApoE） gene polymorphism in stroke patients. Methods Seventy-seven stroke patients of the phlegmdampness constitution were brought into the observation group, eighty stroke patients of not-phlegm-dampness constitution into the control group. The general information of the patients was collected, while their blood lipid level and apolipoprotein level were detected, including triglyceride （TG）, total cholesterol （TC）, high-density lipoprotein cholesterol （ HDL-C）, low-density lipoprotein cholesterol （ LDL-C ）, apolipoprotein A1 （ ApoA1 ） , apolipoprotein B （ApoB） , lipoprotein （a） [ Lp （a） ] , ApoE, as well as the gene polymorphism of ApoE. Results Compared with those in the control group, the TC, TG, LDL-C, ApoE and ApoB levels in the observation group all increased, while the HDL-C level and ApoA1/ApoB decreased （P 〈 0. 05 or P 〈 0.01 ）, and there＇s no significant statistical differ- ences of Lp （a） and ApoA1 levels （P 〉0. 05）. In total three genotypes （e3/e3, e2/e3, e3/e4） and three alleles （ e2, e3, e4） were detected. There was no significant statistical difference in genotype and allele distribution between these two groups by Hardy-Weinberg equilibrium test （P 〉 0. 05 ）, which was consistent with the Hardy-Weinberg equilibrium. The distribution of ApoE genotype and its allele in the two groups were statistically significant （P 〈 0. 05 ）. Compared with the control group, there were more ApoE e4 allele and ApoE e3/e4 genotype in the observa- tion group （P 〈 0.05 ） , while for other genotypes and alleles, there was no significant difference between the two groups （ P 〉 0.05 ）. Conclusion The tendency to stroke among stroke patients of phlegm-dampness constitution might be associated with ApoE e4 allele and ApoE e3/e4 genotype, and the high blood lipid concentration of people with phlegm-dampness constitution might be regulated by ApoE gene polymorphism.