摘要
奶牛乳房炎是危害奶牛业最常见的疾病之一,对奶牛业造成了严重的经济损失。引起奶牛乳房炎的病原菌较多,其中乳房链球菌是常见的致病菌之一。为快速准确地检测样品中的该菌,参照GenBank公布的乳房链球菌pauA基因序列设计引物,建立了乳房链球菌的PCR检测方法。对460份临床奶样进行检测,传统细菌分离方法共检出11份(2.39%)阳性奶样;PCR方法共检出40份(8.70%)阳性奶样,2种方法的吻合率为93.7%。结果显示,建立的乳房链球菌PCR检测法敏感性高,特异性强,适用于临床检测。
Mastitis is one of the most popular diseases that causes significant economic loss in dairy cattle industry. Streptococcus uberisis is one of the pathogens leading to mastitis in dairy cows. According to the sequence of Streptococcus uberis pauA gene published by GenBank, one pair of primers were designed to establish a method for the detection of Streptococcus uberis. And 460 milk samples were detected by this method, a total of 11 （2.39%） of positive milk samples were detected by traditional bacterial isolates, while 40（8.7%） of positive milk samples were detected by PCR, the coincidence rate between the 2 methods was 93.7%. The results showed that the new method is sensitive to detect Streptococcus uberis in clinical milk samples and had the advantages of high sensitivity and good specificity.
出处
《动物医学进展》
北大核心
2017年第8期14-18,共5页
Progress In Veterinary Medicine
基金
广州市科技计划项目(201510010250)
广东省科技计划项目(2014A020208052
2015B020203005
2015A020224039)
广东省兽医临床重大疾病综合防控重点实验室(2013A061401013)
广东省现代农业科技创新联盟建设项目(2016LM2150)
广州市珠江科技新星(201610010073)
关键词
奶牛乳房炎
乳房链球菌
聚合酶链反应
cows mastitis
Streptococcus uberis
polymerase chain reaction
