CD8^+T细胞表位鉴定技术研究进展

Progress on CD8^+T Cell Epitope Mapping Techniques

CD8^+T细胞表位通过MHCⅠ分子呈递到细胞表面,激活CTL细胞从而介导并调节机体抗内源性抗原的免疫应答。MHC-peptide稳定性试验通过TAP缺陷的RMAS细胞转染克隆在细胞水平鉴定CD8^+T细胞表位与MHCⅠ分子的亲和力;酶联免疫斑点技术(ELISPOT)通过检测IFN-γ的分泌水平评估抗原免疫后机体的细胞免疫应答能力,具有较高的敏感性和高效性。四聚体技术通过流式细胞仪分析抗原特异性CD8^+T细胞的表型和功能特征。论文介绍了CD8^+T细胞表位的特征和上述3种表位鉴定方法的基本原理和研究进展,为CD8^+T细胞表位的鉴定和表位疫苗的研制提供参考。

CD8＋T cell epitopes submitted by MHC I molecules can stimulate CTL cells,and play a crucial role in mediating and regulating immunoreactions against endogenous antigens.The binding of peptides to MHCI molecule could be detected using a stably transfected clone of TAP-deficient RMA-S; The IFN- gamma secretory ELISPOT （enzyme-linked immunospot） assay was found to be more sensitive and cost- effective compared to other methods and widely used on the detection of cell immune responses to immunization; Flow cytometry was used to examine the surface markers and biological function of antigen-specific CD8＋ T cell with MHC class I peptide tetramers.This article reviewed the latest progress on the three techniques of experimental identification of CD8＋T cell epitopes and its application for facilitating the development of epitope vaccines.