陕西林麝肺源致病性大肠埃希菌分离鉴定及药敏试验

Isolation,Identification and Drug Susceptibility Test of Pathogenic Escherichia coli from Musk Deer Lungs in Shannxi

为分析致林麝肺炎的病原菌,从发病死亡的林麝肺脏进行细菌分离,对分离所得细菌进行微生物学和生化特性鉴定及动物试验。细菌分离结果显示,病死林麝肺脏主要为2种不同菌落形态的细菌,经挑纯后,测定其16 S rRNA序列,发现2株细菌均为大肠埃希菌,菌株1与Escherichia coli O104:H4序列同源性均为100%,菌株2与Escherichia coli O_(78)序列同源性为100%。用2株菌进行动物试验,发现2株大肠埃希菌对小鼠的致死率均为75%;药敏试验结果表明,头孢类(头孢唑啉、头孢曲松)和单环β内酰胺类(氨曲南)对2株林麝肺部致病性大肠埃希菌均敏感;氨基糖苷类(链霉素)对其均中度敏感;对其他类别抗生素均呈现不同程度的耐药性。

To isolate and identify the pathogenic Escherichia coli from lungs of dead torest musk deer, bacterial culture, biochemical assays and pathogenic test were used. Two different colony morphology of bacteria in forest musk deer were isolated and identified.i6 S rRNA PCR test result showed that two strains of bacteria were Escherichia coil.Strain 1 was 100% similar with E.coli O104：H4, Strain 2 was 100% similar with E.coli O78.The two isolates could cause death in mice.The death rate of mice was 75%.12 kinds of commonly used antibacterials were used to measure their drug sensitivity,cephalosporins have the strongest effect against bacteria among the 12 kinds of antibacterials.Cephalosporins （cefazolin,ceftriaxone） and monocyclic β-lactam （aztreonam） are sensitive to the two strains of pathogenic E.coIi~ aminoglycosides （streptomycin） was moderately sensitive to them; they were resistant to other categories of antibacterials. The findings in the research provided important information for the prevention and control of forest musk deer pneumonia.