miR-218-1-3p对非小细胞肺癌细胞侵袭和迁移影响

Effects of miR-218-1-3p on cell migration and invasion in non-small cell lung cancer

目的非小细胞肺癌(non-small cell lung cancer,NSCLC)因其侵袭性而导致预后不良,miR-218可对多种肿瘤的进展起到抑制作用。本研究探讨miR-218-1-3p对NSCLC A549细胞侵袭和迁移影响。方法使用LipofectamineTM2000Reagent将miR-218-1-3p mimic转染入NSCLC A549细胞中,实时定量聚合酶链反应(real-time PCR,RT-qPCR)检测各组细胞中miR-218-1-3p的表达,Transwell小室法测定其侵袭及迁移能力,荧光定量PCR检测细胞迁移侵袭相关指标基质金属蛋白酶2(matrix metalloproteinase 2,MMP-2)、MMP-7、MMP-9、RhoA、RhoB和RhoC的变化。结果同对照组相比,上调mir-218-1-3p明显抑制了A549细胞的侵袭(t=4.028,P=0.016)和迁移(t=8.911,P=0.001),其侵袭和迁移的抑制率分别为37.8%和53.6%。MMP-7降低至对照组的(0.68±0.19)倍,t=2.931,P=0.043;MMP-9降低至对照组的(0.58±0.15)倍,t=4.875,P=0.080。结论 miR-218-1-3p能够抑制NSCLC A549细胞的侵袭和迁移。

OBJECTIVE Non small-cell lung cancer leads to unfavourable prognosis because of invasion, miR-218 can inhibit the progression of multiple tumors. This research was to investigate the effect of miR-218-1-3p on the invasion and migration of A549 cells in non small cell lung cancer. METHODS miR-218-1-3p was transfected into non small cell lung cancer A549 cells by LipofectamineTM 2000 Reagent, the expression of miR-218-3p was detected by real time PCR. The invasion and migration were assayed by Transwell method. The changes of indicators related to cell migration and in vasion, MMP-2, MMP-7, MMP-9, Rho, A, Rho B, Rho C were detected by fluorescence quantitative PCR. RESULTS Comparing with control group,the cell invasion （t=4. 028,P=0. 016） and migration （t=8. 911,P=0. 001） of A549 were significantly inhibited by up-regulating mir-218-1 3p （P〈0.05）, rating 37.8%and 53. 6%. MMP-7 de creased to （0.68±0.19） times of control group （t=2. 931,P=0. 043） and MMP 9 decreased to （0.58±0.15） times of control group （t=4. 875 ,P=0. 080）. CONCLUSION miR-218-3p can inhibit the invasion and migration of non small cell lung cancer A549 cells.