预防性健康体检中沙门菌检验方法的探导

Study on methods for detection of Salmonella in preventive health examination

目的 探讨一种操作简单、检验成本低、阳性检出率高的预防性健康体检中沙门菌的检测方法.方法 对食品及公共场所从业人员体检1 045人的粪便标本接种氯化镁孔雀绿（MM）增菌液,分别在37 ℃、42 ℃增菌后,再进行平板分离、生化及血清鉴定,比较两种不同温度增菌后沙门菌的阳性检出率.结果 42℃增菌后检出率（2.78%）高于37 ℃增菌检出率（0.57%）,两者之间的差异有统计学意义（χ2=15.371,P〈0.05）.结论 传统的培养法仍是预防性健康体检中沙门菌常用的检测方法,42 ℃增菌后具有更高的检出率,更适用于预防性健康体检中沙门菌的分离鉴定.

Objective To study a method for detecting Salmonella in preventive health examination with simple operation,low inspection cost and high positive detection rate.Methods Fecal specimens were inoculated Rappaport examination of food and public places employees 1 045 people（MM） bacteria,bacteria in the 37 ℃ and 42 ℃ respectively,then the plate separation,and serum biochemical identification,comparison of two different temperature after enrichment of Salmonella positive rate.Results 42 ℃ enrichment rate（2.78%） is higher than 37 DEG C enrichment detection rate（0.57%）,the difference was statistically significant（χ2=15.371,P〈0.05）.Conclusion The traditional training method is to prevent detection of Salmonella in health examination used,42 ℃ enrichment has a higher detection rate,suitable for the isolation and identification of Salmonella in preventive health examination.