一株红树内生真菌Penicillium sp.化学成分研究

Chemical Constituents of a Mangrove-Derived Fungus Penicillium sp.

综合运用各种色谱分离技术硅胶柱层析、Sephadex LH-20凝胶柱层析、高效液相色谱等,从一株来源于药用红树尖瓣海莲(Bruguiera sexangula var.rhynchopetala)的青霉属真菌Penicillium sp.(56211)的发酵产物中分离鉴定了5个化合物.根据理化性质、波谱方法,并与文献数据对照,确定结构分别为:[11]-cytochalasa-6(12),13-diene-1,21-dione-7,18-dihydroxy-16,18-dimethyl-10-phenyl-(7S*,13E,16S*,18R*)(1),[11]-Cytochalasa-6(12),13-diene-1,21-dione-7,18-dihydroxy-16,18-dimethyl-19-methoxy-l0-phenyl-(7S*,13E,16S*,18S*,19R*)(2),[11]-cytochalasa-6(12),13-diene-1,21-dione-7,18,19-trihydroxy-16,18-dimethyl-10-phenyl-(7S*,13E,16S*,18S*,19R*)(3),1-(2,6-dihydroxyphenyl)butan-1-one(4)和O-methylcurvulinic acid(5).所有化合物为首次从尖瓣海莲内生真菌中分离得到.

Column chromatography on silica gel, Sephadex LH-20 and HPLC are used to isolate and purify the metabolites from the fermentation products of Penicillium sp.（56211）, a fungus obtained from a mangrove Bruguiera sexangula var.rhynchopetala. The structures of the isolated compounds are identified by spectroscopic analysis including NMR, and by comparison with the data of literature. Five compounds are isolated from the Et OAc extracts of 56211, and their structures are identified as [11]-cytochalasa-6（12）,13-diene-1,21-dione-7,18-dihydroxy-16,18-dimethyl-10-phenyl-（7S＊,13 E,16S＊,18R＊）（1）, [11]-Cytochalasa-6（12）, 13-diene-1,21-dione-7,18-dihydroxy-16,18-dimethyl-19-methoxy-l0-phenyl-（7S＊, 13 E, 16S＊,18S＊,19R＊）（2）, [11]-cytochalasa-6（12）,13-diene-1,21-dione-7,18,19-trihydroxy-16,18-dimethyl-10-phenyl-（7S＊,13 E,16S＊,18S＊,19R＊）（3）, 1-（2,6-dihydroxy phenyl）butan-1-one（4） and O-methylcurvulinic acid（5）.All compounds are isolated from the fungus, which are isolated from the medicinal mangrove Bruguiera sexangula var. rhynchopetala for the first time.