不同穴位艾灸对原发性痛经大鼠模型NK细胞活性的影响

The Effects of Moxibustion at Different Acupoints on the activity of NK Cells in the Rat Model of Primary Dysmenorrhea

目的:从免疫学角度探讨不同穴位艾灸对原发性痛经大鼠模型NK细胞活性的影响,以期为临床治疗原发性痛经筛选出更好的穴位。方法:利用苯甲酸雌二醇注射液和缩宫素制备大鼠痛经模型,选择治疗痛经的常用腧穴神阙穴和关元穴分别对痛经大鼠进行艾灸治疗,通过观察各组大鼠脾脏重量、扭体次数、脾脏NK细胞活性含量及光镜下脾脏病理切片的变化,比较灸神阙与灸关元治疗原发性痛经的疗效,并从免疫学角度探讨艾灸治疗原发性痛经的机制。结果:(1)与空白组比较,模型组大鼠脾脏重量明显减轻(P<0.01);与模型组比较,各治疗组大鼠脾脏重量均显著增加(P<0.01)。(2)与空白组比较,模型组大鼠扭体次数明显增加(P<0.01);经治疗后,灸神阙组、灸关元组及西药组大鼠扭体次数均明显减少,与模型组比较,差异均有显著统计学意义(P<0.01)。(3)与空白组比较,模型组大鼠脾脏NK细胞活性明显降低(P<0.01);与模型组大鼠脾脏NK细胞活性比较,西药组有统计学意义(P<0.05),灸神阙组与灸关元组均有显著统计学意义(P<0.01)。镜下观察到各治疗组大鼠的脾脏结构均存在,与模型组比较,西药组大鼠脾脏淋巴小结数目及面积稍增多增大,而灸神阙组与灸关元组大鼠脾脏淋巴小结数目及面积均明显增多增大,且灸关元组大鼠脾脏淋巴小结数目稍多于灸神阙组。结论:灸关元穴与灸神阙穴均可通过升高原发性痛经大鼠脾脏NK细胞活性而发挥镇痛作用,但灸关元穴比灸神阙穴对原发性痛经大鼠的免疫影响更明显。

Objective： It is to explore the effect of moxibustion at different acupoints on NK cell activity of the primary dysmenor rhea model in rats from the perspective of immunology so as to select a better acupuncture point in the treatment of primary dysmen- orrhea. Methods. Using rat dysmenorrhea model made of estradiol benzoate injection and oxytocin, we use the moxibustion treat- ment of common primary dysmenorrhea acupoint Shenque and Guanyuan on the dysmenorrhea rats model, respectively. By observing rats＇spleen weight,writhing times, splenic NK cell activity and the content of spleen histopathological changes under light micro- scope, we compare the effect of Shenque and Guanyuan acupoint on primary dysmenorrhea and explore the mechanism of of primary dysmenorrhea from the perspective of immunology. Results ： ①Compared with the blank group, the spleen weight of the model group was significantly reduced （P〈0.01）. Compared with the model group,the weight of the spleen in every treatment group was signifi- cantly increased （P〈0. 01）. ②Compared with the blank group, writhing times in model group increased significantly （P〈0.01 ） ; after treatment,moxibustion Shenque group, moxibustion Guanyuan group and Western medicine group rats＇ writhing times were significantly reduced, compared with the model group （P〈0.01）. ③Compared with blank group, spleen NK cell activity in the model group decreased significantly （P〈0.01） ; compared with the splenic NK cell activity in the model group, the western medicine group has difference （P〈0.05） ; moxibustion Shenque group and moxibustion Guanyuan group have extremely significant difference （P〈 0.01）. Under the microscope observation,the spleen structure in each treatment group rats was found. Compared with the model group, the spleen lymphoid nodule number and area in western medicine group rats increased or enlarged slightly; rats spleen lymph- oid nodule number and area in the moxibustion Shenque group and moxibustion Guanyuan group were significantly increased in number and size; the spleen lymphoid nodule number in moxibustion Guanyuan is slightly more than that in the moxibustion Shenque group. Conclusion： Moxibustion on Guanyuan and Shenque can play analgesic effect by increasing splenic NK cell activity in prima- ry dysmenorrhea, but the effect of moxibustion Guanyuan on primary dysmenorrhea is more obvious than that in moxibustion Shenque.