摘要
为了准确、迅速地筛查并确定酸枣仁中微量生物碱成分,运用含氮有机物(生物碱)分析包中的色谱柱建立酸枣仁生物碱成分的高效液相色谱在线分离方法,然后利用Mass Works分子识别软件和LC-ESI-MS/MS对在线分离的生物碱成分进行确认,共筛查到3种阿朴芬类生物碱:酸枣仁碱K(C_(17)H_(19)NO_3)、山矾碱(C_(17)H_(17)NO_2)和N-甲基巴婆碱(C_(18)H_(19)NO_2);2种环肽类生物碱:酸枣仁碱F(C_(31)H_(42)N_4O_5)和酸枣仁碱A(C_(31)H_(42)N_4O_4);还有一对同分异构体阿朴芬生物碱:木兰花碱和酸李碱(C_(20)H_(24)NO_4)有待进一步确定。
To accurately and quickly screen and identify trace alkaloids components from Semen Zizyphi spinosae, a HPLC online separation method of alkaloids components was developed using the columns which belong to the nitrogen- containing organic compounds （alkaloids） analysis kit. Then the alkaloids components separated on line were identified with the help of MassWorks molecular recognition software and LC-ESI-MS/MS. Three compounds ： sanjoinine K （C17H19NO3 ） ,caaverine （ C17H17NO2 ） and N-methylasimilobine （ C18H19NO2 ）, belong to aporphine alkaloids and two compounds：sanjoinine F （C31H42N4O5 ） and sanjoinine A （C31H42N4O4 ）, belong to cyclopeptide alkaloids were screened and identified. In addition, there was a pair of isomeric compounds ： magnoflorine and zizyphusine （ C20H24NO4 ）, which belong to aporphine alkaloids to be further identified. A rapid and efficient screening method for alkaloids from Chinese medicine was established in this study.
作者
胡彦周
丁轲
韩涛
陈湘宁
HU Yan-zhou DING Ke HAN Tao CHEN Xiang-ning(College of Food Science and Engineering, Bering University of Agriculture, Beifing Food Quality and Safety Laboratory,Microbiological and Safety Inspection and Control of Pesticide Residues in Agricultural Products Harmful Beijing Key Laboratory,Beijing 102206, China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2017年第7期1165-1170,共6页
Natural Product Research and Development
基金
北京市属高等学校高层次人才引进与培养计划(CIT&T CD20154045)
北京农学院青年基金--实验技术体系研发基金项目(SXST201604)
北京市自然科学基金委员会--北京市教育委员会联合资助项目(KZ201710020014)
2016年北京农学院学位与研究生教育改革与发展项目(2016YJS051)
