叶酸对PCPA抑郁小鼠外侧缰核β-CaMKⅡ水平的影响

Effects of Folic Acid on the Level of β-CaMK Ⅱ in Lateral Habenular Nucleus of PCPA Depression Mice

为探究叶酸对PCPA抑郁症模型小鼠的干预作用及其对外侧缰核β-Ca MK II水平的影响,将昆明种小鼠40只随机分为三组:对照组、模型组、叶酸组,通过腹腔注射PCPA(100 mg/kg)每日1次,连续4 d构建抑郁症模型,旷场与悬尾实验观察小鼠行为变化,HPLC检测小鼠下丘脑5-HT含量,免疫组化测定小鼠外侧缰核β-Ca MK II表达水平。结果显示,模型组与对照组相比自主活动能力下降,悬尾不动时间延长(P<0.05),而叶酸组与对照组相比上述实验结果无统计学差异(P>0.05)。模型组与对照组相比下丘脑5-HT含量降低,外侧缰核β-Ca MK II表达水平下降(P<0.05)。结果表明叶酸能显著提高下丘脑5-HT含量与外侧缰核β-Ca MK II水平,其抗抑郁作用机制可能与之有关。

To assess the effects of folic acid and the levels of β-CaMK II in lateral habenular nucleus of PCPA depression mice. 40 Kuming mice were randomly divided into control group, model group and folic acid group. The mice were intraperitoneally injected by PCPA once a day over a period of 4d to construct depression model. Then the changes in behaviors were observed. The level of 5-HT in the hypothalamus was detected by HPLC and the expression of β-CaMK II in the lateral habenular nucleus was verified by immunohistochemistry. Compared with model group, the locomotor activity was significantly increased （P 〈 0.05） and the immobility time of tail suspension was obviously reduced in folic acid treatment groups （P 〈 0.05）. The level of 5-HT in the hypothalamus was detected by HPLC and the expression of β-CaMK II in the Lateral habenular nucleus were significantly decreased （ P 〈 0.05 ）. The results indicated that folic acid can significantly increase hypothalamus 5-HT and lateral habenular nucleus β-CaMK II,which could be the mechanism of antidepressant effect of folic acid.