板蓝根正丁醇部位抗病毒活性组分及相关化学成分研究

Antiviral activity and chemical composition of n-butanol extract of Isatidis Radix

目的研究板蓝根Isatidis Radix正丁醇部位的抗病毒活性组分及相关成分。方法利用溶剂法及色谱法提取分离板蓝根正丁醇部位各化学组分,MTT法体外筛选各组分抗单纯疱疹病毒1(HSV-1)活性,利用UPLC-Q-TOF-MS检测正丁醇部位及各组分的化学成分,运用Markview软件及Excel软件进行各组分差异性成分分析,根据统计结果得到抗病毒相关的化学成分。结果按照化学成分的相似性,利用色谱法将板蓝根正丁醇部位分为20个组分,其中10个组分具有明显抗病毒活性。UPLC-Q-TOF-MS检测到正丁醇部位中的44个化学成分,并明确了它们的化学结构,在各化学组分的分析检测及差异性分析中,发现了生物碱类、有机酸、糖和氨基酸衍生物共15个化学成分与抗病毒活性相关;尤其是1-甲氧基-3-甲醛、3-(3′,5′-二甲氧基-4′-羟基)-2-吲哚酮、1-甲氧基-3-吲哚酮、3-(甲基硫代)芥子油苷、氨基葡萄糖-6-磷酸酯在活性组分中量较高。结论板蓝根正丁醇部位以吲哚类生物碱、有机酸、核苷及氨基酸类成分为主,其中的单吲哚类、硫苷类及糖和氨基酸衍生物与抗病毒活性密切相关。

Objective To investigate the antiviral activity and chemical composition of n-butanol extract of Isatidis Radix. Methods Each component of total n-butanol extracts was detected by solvent and chromatogram, the anti-HSV-1 activities of each component were detected by MTT assay in vitro, each component of total n-butanol extracts was analyzed by CUPLC-Q-TOF-MS, and the data were analyzed by Markview and Excel software to find the difference among different components. According to statistics, the components related to antivirus activities were obtained. Results According to the similarity of chemical composition, 20 components were extracted and separated from n-butanol extract of Isatidis Radix by chromatography, and 10 components had antiviral activity. Forty-four chemical components were detected by UPLC-Q-TOF-MS from n-butanol extracts and their chemical structures were confirmed. Difference analysis of chemical components discovered 15 chemical components such as alkaloids, organic acids, sugars and amino acid derivatives associated with antiviral activity, especially 1-methoxy-3-carbaldehyde, 3-（3′,5′-dimethoxy-4′-hydroxy）-2-indolinone, 1-methoxy-3-indolinone, 3-（methylsulfinyl） propylglucosinolate, and glucosamine-6-phosphatee in higher levels of the active components, which was worth further study. Conclusion The n-butanol extract of Isatidis Radix mainly contains indole alkaloids, organic acids, nucleosides and amino acids, wherein the single-indole, a sulfur glycosides and sugars and amino acid derivatives are closely related to the antiviral activity.