乌头自然群体株系的第一、二子根质量标记-性状关联分析

Marker-trait association analysis for weight of first and second fleshy root in 126 diverse lines of Aconitum carmichaelii

目的发掘乌头子根质量相关分子标记,为乌头新品种选育提供技术支撑。方法研究利用280个AFLP分子标记位点对126份乌头的自然群体进行了第一、二子根质量的分子标记-性状关联分析。结果 STRUCTURE软件基于k值变化推导出供试自然群体的最佳亚群数为2,关联分析识别到19个目标性状关联位点,分别解释了0.87%~6.69%的表型变异。其中P1M1-27、P3M3-30及P1M1-20在GLM模式、MLM模式、GLM+K模式及GLM+Q模式中均被识别到。结论研究发掘到的目标性状关联位点对乌头分子标记辅助育种具有重要意义。

Objective To identify molecular markers associated with weight of fleshy root of Aconitum carmichaelii, and provide technologic basis to A. carmichaelii breeding. Methods The marker-trait association（MTA） analysis for the weight of first and second fleshy root was conducted in association mapping panel comprised 126 diverse lines based on 280 AFLP loci. Results The population structure analysis of all lines conducted using STRUCTURE indicated that the likely number of subgroups was 2 based on the change in k. A total of 19 loci were identified to associate with 0.87%—6.69% of phenotypic variance for objective trait. The loci of P1M1-27, P3M3-30, and P1M1-20 were identified under all analysis modes of GLM, MLM, GLM ＋ K, and GLM ＋ Q. Conclusion These loci should be compatible to marker assistance selection during A. carmichaelii breeding.