基于ISSR分子标记的郁金香品种遗传多样性分析

Genetic Diversity Analysis of Tulip Varieties Based on ISSR Markers

本研究利用ISSR分子标记技术对22个郁金香品种进行遗传多样性分析,以期了解郁金香不同品种之间的遗传关系,提高育种的目标性。利用11条引物共扩增出129条谱带,其中多态性谱带为124条,多态性率为96.1%,22个郁金香品种的检测等位基因数(na*)平均为1.983 9,有效等位基因数(ne*)平均为1.383 3,Nei's基因多样性指数(h*)平均为0.249 0,Shannon's信息指数(I*)平均为0.399 0,表明郁金香多态性较高,具有丰富的遗传多样性。利用NTSYS软件进行UPGMA聚类分析,22个品种在遗传相似系数0.62为阈值处可分为两大类,根据聚类结果,理论上推测不同花色的品种间杂交获得新品种的潜力较大。此结论为进一步研究和利用郁金香提供分子水平的依据。

In this study, the genetic diversity of 22 tulip varieties was analyzed based on ISSR molecular markers, aiming to understand the genetic relationship between different varieties of tulips and improve the goal of breeding. 129 bands were amplified by use of 11 primers, in which 124 were polymorphic bands and the polymorphism rate was 96.1%. The average number of tested alleles （na＊）, effective alleles （ne＊）, Nei＇s gene diversity index （11＊） and Shannon＇s information index were 1.793 9, 1.383 3, 0.249 0, and 0.399 0, respectively, which indicated that the polymorphism of tulip was high, and it had rich genetic diversity. UPGMA cluster analysis done by NTSYS software showed that 22 species could be classified into two cluster groups with the similarity coefficient of 0.62. According to the clustering results, it was speculated that the hybridization of varieties of different colors had greater potential to get new varieties. This conclusion could provide theoretical reference at the the molecular level for further research and utilization.