壳聚糖絮凝耦合糖析萃取葡萄籽中的活性成分

Chitosan-Based Flocculation Coupled with Sugaring-out Extraction of Active Ingredient from Grape Seeds

采用壳聚糖絮凝剂纯化微波辅助提取的葡萄籽水提液,考察了壳聚糖浓度、p H、絮凝时间、料液比对葡萄籽水提液活性成分纯化效果的影响,并结合正交试验优化参数。所得数据表明:按照料液比为1:15(g/m L)制得的葡萄籽水提液,调节p H为6,加入壳聚糖使其终浓度为0.8 mg/m L,絮凝时间为60 min,葡萄籽中原花青素、总黄酮、总多酚、齐墩果酸的回收率分别为85.4%、88.3%、84.4%、85.5%。在44%(w/w)叔丁醇/20%(w/w)葡萄糖糖析萃取体系中,加入絮凝后葡萄籽水提液使其质量分数为22%(w/w),加水补足至100%。原花青素、总黄酮、总多酚、齐墩果酸的分配系数分别为2.56、2.36、2.49、2.34;萃取率分别为87.6%、86.69%、87.3%、86.59%。

Aqueous extract of grape seeds was obtained by microwave assisted chitosan flocculant purification. The effect of the chitosan concentration, pH, flocculation time and solid-solvent ratio on purification were investigated, and the optimum conditions were obtained by orthogonal experiments. The results showed that the optimum conditions used the aqueous extract of grape seeds whose solid-solvent ratio was 1：15 （g/mL）. Its pH was adjusted to 6, and the final concentration of the aqueous extract was 0.8 mg/mL after adding chitosan flocculants where the flocculation time was 60 min. Under the optimum conditions, the recovery rate of procyanidins, total flavonoids, total phenolics, and oleanolic acid were 85.4%, 88.3%, 84.4% and 85.5%, respectively. In the sugaring- out extraction system composed of 44% （w/w） t-butanol and 20% （w/w） glucose, the mass fraction of aqueous extract of grape seed became 22% （w/w） by flocculating. Then, we added distilled water to finally obtain 100% concentration of the system. The distribution coefficients of procyanidins, total flavonoids, total phenolics, and oleanolic acid were 2.56, 2.36, 2.49 and 2.34, while their extraction rates were 87.6%, 86.69%, 87.3% and 86.59%.