周易象数切脉疗法对功能性消化不良患者Ghrelin、GHS-R分子表达变化的影响

Effect of Zhouyi Xiangshu Qiemai therapy on expression of ghrelin and GHS-R in gastric mucosa of patients with functional dyspepsia

目的观察周易象数切脉疗法对功能性消化不良(functional dyspepsia,FD)患者血清胃促生长素(Ghrelin)释放及胃体黏膜Ghrelin、生长激素促分泌物受体(growth hormone secretagogue recepter,GHS-R)分子表达变化的影响.方法观察对象为80例FD合格受试者.治疗组40例行周易象数切脉疗法治疗,莫沙必利西药组40例予枸橼酸莫沙必利分散片口服治疗,检测2组治疗前、治疗第7、14、21天血清Ghrelin的变化情况;同时检测治疗前后胃体黏膜Ghrelin、GHS-R分子表达变化.结果治疗前,血清Ghrelin、胃体黏膜Ghrelin、GHS-R比较,差异无统计学意义(P>0.05);治疗后,2组血清Ghrelin含量、胃体黏膜Ghrelin、GHS-R与治疗前比较增高显著,差异有统计学意义(P<0.05);治疗后2组同一时间点Ghrelin含量比较,差异有统计学意义(P<0.05);采用Spearman相关性分析,结果显示,血清Ghrelin与胃体黏膜Ghrelin、GHS-R表达呈正相关(P=0.002,P=0.001).结论周易象数切脉疗法对功能性消化不良患者良好的临床疗效可能与上调胃肠激素Ghrelin释放及胃体黏膜Ghrelin、GHS-R表达升高相关.

AIM To observe the effect of Zhouyi Xiangshu Qiemai therapy on the expression of ghrelin and growth hormone secretagogue recepter （GHS-R） in the gastric mucosa of patients with functional dyspepsia （FD）. METHOD8 Eighty FD patients were divided into either an observation group or a control group, with 40 patients in each group. The observation group was treated with Zhouyi Xiangshu Qiemai therapy, and the control group was treated with mosapride citrate dispersible tablets. Serum levels of ghrelin were detected before and 7 d, 14 d and 21 d after treatment. The expression of ghrelin and GHS-R in the gastric mucosa was also detected. RESULTS Before treatment, serum level of ghrelin and the expression of ghrelin and GHS-R in the gastric mucosa did not differ significantly between the two groups （P 〉 0.05）. After treatment, serum levels of ghrelin and the expression of ghrelin and GHS-R in the gastric mucosa in both groups were significantly higher than those before treatment （P 〈 0.05）, and serum level of ghrelin differ significantly between the two groups at the same time points （P 〈 0.05）. Spearman correlation analysis showed that serum ghrelin was positively correlated with ghrelin and GHS-R expression in the gastric mucosa （P = 0.002, P = 0.001）. Zhouyi Xiangshu Qiemai therapy exerts its therapeutic effect in patients with functional dyspepsia possibly by up-regulation of ghrelin release and ghrelin/GHS-R expression in the gastric mucosa.