茵栀黄对结构性雄甾烷受体CAR介导CYP3A4的转录调节作用

TRANSCRIPTIONAL REGULATION OF YINZHIHUANG ON CYP3A4 MEDIATED BY STRUCTURAL MALE STEROID RECEPTOR CAR

目的:探讨茵栀黄对CYP3A4转录表达的影响及机制。方法:Chang Liver细胞中加入0.01、0.1和1μM茵栀黄分别作用24h、36h和48h,采用RT-PCR法和报告基因方法检测CYP3A4 m RNA的表达水平。结果:不同浓度茵栀黄分别处理Chang Liver细胞24h和36h可明显升高CYP3A4 m RNA的表达水平(P<0.05);转入CAR载体的Chang Liver细胞经不同浓度茵栀黄处理36h均可激活CYP3A4的转录表达。结论:茵栀黄可能通过CAR介导增加Chang Liver细胞CYP3A4的转录表达。

Objective：To investigate the effects and mechanisms of Yinzhihuang on transcription of CYP3A4. Methods： The Chang Liver cells were cultmed with 0.01μM, 0.1μM, 1μM Yinzhihuang respectively for 24h, 36h and 48h. The expression of CYP3A4 mRNA was detected by RT-PCR and dual-lucifemse reporter assay. Results： The Chang Liver cells were cultured with Yinzhihuang of different concentration for 24h and 3611 could obviously increase the expression of CYP3A4 mRNA （P（0.05）. The Chang Liver cells cells Wansfected CAR vector were cultured with different concentrations of Yinzhihuang for 36h could activate transcription of CYP3A4. Conclusions： Yinzhihuang may increase the transcriptional expression of CYP3A4 in Chang Liver cells mediated by CAR.