4种保存液对犬红细胞低温保存效果的比较

Effects of four different preservation solutions on low-temperature preservation of canine erythrocyte

犬类红细胞低温保存具有较大的临床应用价值,犬红细胞低温保存液对犬红细胞低温保存的时间和保存效果具有重要影响,该实验选用CPDA-1、CPDA-2、CPDA-3和MAP保存液对犬红细胞进行4℃下低温保存,于保存的第0、10和20天分别检测各组红细胞样品的红细胞数及K^+、Na^+和Cl^-的浓度,对低温保存效果进行比较,以寻找适合犬红细胞低温保存的保存液。结果表明:选用MAP保存液对犬红细胞进行保存时,红细胞相对减少量显著低于其它组(P<0.05),红细胞样品中K^+浓度的相对增加量显著低于其它组(P<0.05),Na^+和Cl^-浓度的相对减少量显著低于其它组(P<0.05),说明MAP保存液较适合用于犬红细胞的低温保存。

Low-temperature preservation of canine erythrocyte has important clinical value,the preservation solution plays an important role in canine erythrocyte preservation.In this study,four different preservation solutions（CPDA-1,CPDA-2,CPDA-3and MAP）were used for canine erythrocyte preservation at 4 ℃,the proper preservation solution was selected according to the erythrocyte number and K~＋,Na~＋ and Cl^- concentrations.The results showed that the erythrocyte number in preservation solution was decreased,and the reduction number of erythrocyte in MAP group was significantly lower than that of other groups（P〈0.05）.The K＋concentration in preservation solution was increased,but the K^＋ augmenter in MAP group was significantly lower than that of other groups（P〈0.05）.The Na^＋/Cl^- concentration in preservation solution was decreased,but the Na^＋/Cl^- concentration decrements in MAP group was significantly lower than that of other groups（P〈0.05）.These results indicated the MAP solution was more suitable for the low-temperature preservation of canine erythrocyte.