基于肥大细胞活化探讨温阳固卫方治疗慢性荨麻疹的机制

Based on Activation of Mast Cells to Explore the Mechanism by which Warming Yang and Defensing Qi Improves Chronic Urticaria

目的研究温阳固卫方对阳虚大鼠被动过敏实验的影响,探讨温阳固卫方对慢性荨麻疹肥大细胞膜稳定性的影响。方法通过构建类阳虚大鼠被动皮肤过敏(passive cutaneous ana-phylaxis,PCA)模型,观察温阳固卫方对类阳虚PCA大鼠颅骨骨膜肥大细胞活化的影响。结果各组实验大鼠颅骨骨膜肥大细胞脱颗粒率显示:与空白组比较,中药+西药组、中药组、西药组均可减少肥大细胞脱颗粒的发生,差异具有统计学意义(P<0.05);其中中药+西药组肥大细胞脱颗粒率明显减少(P<0.05);中药组和西药组在肥大细胞脱颗粒减少方面差异无统计学意义(P>0.05)。结论温阳固卫方具有抑制皮肤Ⅰ型超敏反应的作用,能抑制大鼠异种皮肤被动过敏反应;对其脱颗粒的肥大细胞具有抑制作用。

Objective To study the influence of warming yang and defensing qi on allergic response in rats with yang deficiency, and to assess the effect of a formula for warming yang and defensing qi on the stability of mast cell membrane in rats with chronic urticaria. Methods Passive cutaneous anaphylaxis（PCA）model was established in rats with yang deficiency, followed by assessment of the effect of a formula for warming yang and defensing qi on activation of mast cells from the periosteum of the skull. Results In comparison with the controls,all treatments dramatically reduced the degranulation rate of mast cells from the periosteum of the skull（P〈0.05）. A more dramatic reduction in the degranulation rate was observed in the group-treated with Chinese medicines plus western medicines.However, reduction in degranulation rates of mast cells were no significant differences between group-treated with Chinese medicines and with western medicines.Conclusion The formula for warming yang and defensing qi can inhibit type Ⅰ cutaneous hypersensitivity,passive cutaneous anaphylaxis and the degranulation of mast cells.