血浆miR-1、miR-133a以及miR-126—急性心肌梗死的新型生物学标记物

Plasma miR-1,miR-133a,and miR-126 as Novel Biomarkers of Acute Myocardial Infarction

目的:探讨急性心肌梗死(AMI)早期血浆miR-1、miR-133a以及miR-126的水平变化,与血浆cTnⅠ的相关性,及三者是否可作为早期诊断AMI标记物。方法:应用real-time PCR方法,检测24位AMI患者以及8名对照者血浆miR-1、miR-133a以及miR-126的含量,运用ELISA方法检测血浆肌钙蛋白I(cTnⅠ)浓度。ROC曲线分析上述三种血浆miRNAs在AMI中的诊断价值及意义,并分别与cTnⅠ相对比。结果:三种循环miRNAs在AMI患者中均存在显著差异性表达,其中AMI患者血浆miR-1的含量较对照组增高约46.6±11.9倍(P=0.001),血浆miR-133a的浓度较对照组增高39.9±9.5倍(P<0.05)。AMI患者血浆miR-126的水平较对照组显著降低,约为对照组的0.11±0.01倍(P<0.05)。关联性分析表明血浆miR-1以及miR-133a的含量与cTnⅠ呈显著正相关;ROC曲线分析发现,三者均有显著的诊断价值,其中以低表达的miR-126诊断效力最强(AUC:1.000,95%可信区间为0.000~1.000)。联合miR-1和miR-133a显著提高二者的诊断效能(AUC:0.964,95%可信区间为0.000~1.000)。结论:AMI早期血浆miR-1、miR-133a以及miR-126水平变化显著,与血浆cTnⅠ相关性好,可作为早期诊断AMI标记物,具有临床应用前景。

Objective： To study the changes of miR-1,miR-133 a,and miR-126 in the early acute myocardial infarction patients,the correlation of the three of them with plasma cardiac troponin I（ cTnⅠ）,and powerful biomarkers for the early diagnosis of acute myocardial infarction. Methods： Total 24 patients with acute myocardial infarction（ AMI）were recruited in this study. The expressions of circulating miR-1,miR-133 a,and miR-126 were detected in 24 AMI patients and 8 healthy people as control group by real-time PCR. Plasma cardiac troponin I（ cTnⅠ） concentrations were measured by ELISA. The receiver operating characteristic（ ROC） curve was used to assess the diagnostic value of these three circulating miRNAs for diagnosing AMI. Results： According to the study,there was significantly differential expression observed in these three miRNAs,in which the expressions of miR-1（ 46. 6 ± 11. 9 fold）（ P = 0. 001） and miR-133a（ 39. 9 ± 9. 5 fold）（ P〈0. 05） were remarkably increased in AMI patients,whereas the concentration of miR-126（ 0. 11 ± 0. 01 fold）（ P〈0. 05） was reduced. The correlation analysis showed that plasma levels of miR-1and miR-133 a were positively correlated with plasma cTnⅠ. ROC analysis revealed that all of these three circulating miRNAs had significant diagnostic value. Among Which,the diagnostic power of miR-126 was the strongest（ AUC： 1.000,95% confidence interval 0. 000-1. 000）. The combination of miR-1 and miR-133 a significantly improved the diagnostic accuracy（ AUC： 0. 964,95% confidence interval 0. 000-1. 000）. Conclusions： Circulating miR-1,miR-133 a,and miR-126,the correlation of which with plasma cardiac troponin I（ cTnⅠ） has been proved,can be potential and powerful biomarkers for early diagnosis of acute myocardial infarction.