UPLC-MS/MS法测定电子烟烟油中TSNAs的含量

Determination of Tobacco-specific N-nitrosamines (TSNAs) Content in Electronic Cigarette Liquid by Using UPLC-MS/MS Method

为快速准确测定电子烟烟油中的烟草特有亚硝胺(TSNAs),对前处理条件进行优化,建立了烟油中TSNAs的超高效液相色谱-串联质谱(UPLC-MS/MS)方法,结果表明:使用HLB固相萃取小柱进行净化,可以很好地去除杂质干扰;NNK、NNN、NAT、NAB的检出限为0.005~0.020 ng/g,定量限为0.015~0.067 ng/g,3个加标水平的回收率为85.16%~105.79%;通过对6个不同品牌电子烟烟油中TSNAs的测定,NNN的检出率和含量是最低的,因此,该方法稳定、准确,适用于测定电子烟烟油中TSNAs的含量。

An improved method was developed to rapidly and accurately determine four kinds of tobacco-specific N-nitrosa- mines （TSNAs） in electronic cigarette liquid by using uhra-performance liquid chromatography-tandem mass spectrometry （UP- LC-~/IS/MS） method. The results showed that using I-ILB solid-phase extraction column could well purify the TSNAs in electronic cigarette liquid. The limit of detection for NNK, NNN, NAT and NAB in samples was 0.005- 0.020 ng/g, and the limit of their quantification ranged from 0.015 ng/g to 0.067 ng/g. The recovery of 3 analytes varied from 85.16% to 105.79%. This method was used for the determination of TSNAs content in the liquid of 6 brands of electronic cigarette, and it was found that both the de- tection rate and the content of NNN were the lowest. Therefore, this method is stable and accurate, and it is suitable for the deter- mination of TSNAs content in liquid of electronic cigarette.