玉米大斑病菌StSte12基因对H_2O_2氧化胁迫的应激调节

Regulation of StSte12 on H_2O_2 Oxidative Stress in Setosphaeria turcica

通过比较野生型菌株Wt01-23与StSte12基因RNAi沉默突变体菌株StRNAi9-10和StRNAi3-6在H_2O_2胁迫下生长和发育方面的差异,分析转录因子基因StSte12对玉米大斑病菌氧化胁迫的调节能力。在不同浓度H_2O_2胁迫条件下,测定野生型菌株和突变体菌株的菌落生长速度、菌丝形态、产孢量和菌丝萌发率。结果表明,随着H_2O_2浓度的增加,玉米大斑病菌野生型菌株和突变体菌株的菌落生长速度、产孢量和菌丝萌发率均显著降低,但突变体菌株的降低程度显著高于野生型菌株,表明StSte12基因对玉米大斑病菌的氧化应激调节具有重要的调控功能。

The ability of transcription factor StSte12 regulating oxidative stress in Setosphaeria turcicawas studied with the wildtype Wt01-23 and RNAi mutants（StRNAi 9-10, StRNAi 3-6）. Under the stress of H2O2, the colony growth rate, hyphal morphology, conidiation and mycelium germination of the three isolates were observed. The resuhs showed that the colony growth speed, conidiation and mycelium germination were significantly inhibited for all the three isolates, but the inhibition efficiency of RNAi mutants were significantly higher than the wild type. The experimental results supported the important regulation function of StStel2 on the oxidative stress of S. turcica.