酸热辅助超声法提取类球红细菌发酵产物CoQ_(10)

Extraction of Coenzyme Q_(10) from Rhodobacter sphaeroides with Acid Heating-Assisted Ultrasonication

以类球红细菌为试验菌株,对其发酵制备的辅酶Q_(10)进行提取。通过比较醇碱皂化法、超声波破碎法和酸热辅助超声法三种不同破胞方法,确定利用酸热辅助超声法提取发酵液中的辅酶Q_(10)。并对酸热辅助超声法进行单因素和正交试验,优化了类球红细菌辅酶Q_(10)的提取工艺,得出的最佳工艺条件为:盐酸300μL,温度80℃,超声波破碎输出功率400 W,工作时间20 min。在此条件下,辅酶Q_(10)得率可达3.738 mg/g。

Rhodobacter sphaeroides was used as the test strain, and coenzyme Q10 of the strain was extracted. Compared with three different methods of saponification, ultrasonication and acid heating-assisted ultrasonication, extraction of coenzyme Q10 from R.sphaeroides with acid heating-assisted ultrasonication was determined. Extraction technology of coenzyme Q10 was optimized by single factor and orthogonal test. The optimal condition for coenzyme Q10 extraction was as follows： HCl 300 μL, temperature 80 ℃, output power 400 W, working time 20 min. Under these conditions, yield of coenzyme Q10 could reach to 3.738 mg/g.