通腑活血方对大鼠术后肠粘连的影响

Influence of Tongfu Huoxue Prescription over Postoperative Intestinal Adhesion

目的通过制作术后肠粘连SD大鼠模型,证实通腑活血方能有效防治术后肠粘连的发生,并初步探讨其作用机制。方法 SD大鼠40只,随机分为4组,即正常组(n=10)、模型组(n=10)、大黄虫丸组(n=10)和通腑活血方组(n=10),术后第7天观察各组大鼠肠粘连等级,并采用ELISA法检测各组大鼠IL-6、TNF-α血清浓度,同时在X线和B超下观察其肠腔扩张及血运情况。结果大黄庶虫丸组和通腑活血方组肠粘连等级均低于模型组(P<0.05),且术后第7天血清IL-6和TNF-α水平均低于模型组(P<0.01);X线和B超观察发现:通腑活血方组较模型组肠管血供较好,肠管蠕动更频繁且肠腔积气明显减少。结论通腑活血方能够降低血清中IL-6和TNF-α水平、促进肠道蠕动、减轻肠管扩张,从而防治大鼠术后肠粘连的发生。

Objective： To prove that Tongfu Huoxue Prescription can effectively prevent postoperative intestinal adhesion and primarily explore its mechanisms by preparing the SD rats model with postoperative intestinal adhesion. Method： Totally 40 rats were randomly divided into 4 groups ： normal, model, Dahuangchong Pill and Tongfu Huoxue Prescription group with equal numbers. On the 7th day after operation, the adhensive degrees in all groups were defined, the serum IL-6 and TNF-α concentrations were checked by ELISA and the conditions of intestinal distension as well as blood circulation were observed under X-ray and ultrasound. Results： The adhesive degrees in Dahuangchong Pill and Tongfu Huoxue Prescription group were lower than in the model group （P〈0.05） and the IL-6 and TNF-α levels in these two groups were lower than the model group on the 7th day after operation （P〈0.01）. X-ray and ultrasound results： The blood circulation of Tongfu Huoxue Prescription group was better, the bowel movements were more frequent and air accumulating in the cavity significantly reduced, compared with the model group. Conclusion： Tongfu Huoxue Prescription is able to decrease the IL-6 and TNF-α levels, promote bowel movement and lighten intestinal distention to prevent the occurrence of rats＇ postoperative intestinal adhesion.