三七主要皂苷类药效成分含量的近红外光谱测定法

Rapid Quantitative Determination of Main Saponin Constituents in Panax Notoginseng by Near-infrared Spectroscopy Technique

目的建立基于近红外光谱快速测定三七药材中5种主要皂苷类药效成分含量的方法。方法取173批不同部位、不同产地、不同大小规格的三七药材,采用HPLC定量分析方法测定三七皂苷R_1、人参皂苷Rg1、Re、Rb_1和Rd的含量,作为参考值。药材粉碎后在4 000~10 000 cm^(-1)波数范围内采集光谱,对光谱预处理方法、建模波段及主成分数进行优选,采用偏最小二乘回归算法建立近红外光谱与三七药材中5种主要药效成分含量HPLC分析结果之间的多元校正模型。结果三七皂苷R_1、人参皂苷Rg1、Re、Rb_1和Rd在校正模型中的预测相关系数(r)分别为0.959 6,0.978 5,0.902 6,0.966 0和0.992 9。结论该方法测定的5种皂苷是三七的主要药效成分,且为三七总皂苷类制剂的质量检测指标。本方法操作简便快速,结果准确,可用于三七药材质量的快速检测。

OBJECTIVE To establish a rapid quantitative method for determining main saponin constituents in Panax notoginseng by near-infrared spectroscopy（NIR）. METHODS A total of 173 batches of Panax notoginseng samples with different sizes, from different parts and geographical regions were collected, and the contents of notoginsenoside R_1, ginsenoside Rg1, Re, Rb_1 and Rd as reference were quantitatively determined by HPLC. The crushed samples of Panax notoginseng were used to obtain the NIR spectra of 4 000-10 000 cm^（-1）. Model parameters including pretreatment of spectra, wavelength range and PLS factors were optimized. Partial least squares regression（PLSR） was employed to establish the calibration models between the spectra and the contents of main active constituents, respectively. RESULTS In the constructed PLS models, the values of correlation coefficient（r） of notoginsenoside R_1, ginsenoside Rg1, Re, Rb_1 and Rd were 0.959 6, 0.978 5, 0.902 6, 0.966 0 and 0.992 9, respectively. CONCLUSION The constituents determined by this method are the main active constituents of Panax notoginseng, which are also the quality assessment markers for the preparations derived from the total saponins of Panax notoginseng. The proposed method is rapid, convenient and accurate, which can be used for the rapid analysis of the main active constituents in Panax notoginseng.