茯贞膏对贫血小鼠造血功能影响的研究

Study on Effects of Fuzhen Ointment on Anemia of Hematopoiesis in Mice

目的:探讨茯贞膏对贫血小鼠造血功能的影响。方法:选取128只BALB/c小鼠作为本次实验的研究对象,首先适应性喂养小鼠1周,然后运用物理辐射方法将小鼠进行环磷酰胺致贫血造模,造模工程结束后,将128只BALB/c小鼠按照随机数表法平均分为观察组与对照组,观察组64只小鼠,对照组64只小鼠。对两组小鼠采取不同的给药方式,给予观察组小鼠茯贞膏灌位给药,每日给药9 g/kg,9日为1个周期,连续给药1个周期;对照组给予生理盐水灌胃给药,服用剂量与观察组相同,给药周期为9 d。对两组小鼠治疗9 d后血象、骨髓有核细胞计数和脾脏指数变化情况等相关指标进行比较分析。结果:观察组小鼠治疗后RBC数值为(9.6±1.5)×10^(12)/L,对照组RBC数值仅为(7.9±1.8)×10^(12)/L,观察组RBC数值明显高于对照组数值,二者差异明显,具有统计学意义(P<0.05);观察组小鼠治疗后WBC数值为(19.7±1.3)×10~9/L,对照组WBC数值为(6.1±1.9)×10^(12)/L,对照组明显高于观察组,二者有明显差异,具有统计学意义(P<0.05);观察组小鼠治疗后HB数值为(199.5±5.6)g/L,明显高于对照组的(159.1±4.8)g/L,二者差异明显,具有统计学意义(P<0.05);观察组小鼠治疗后PLT数值为(488.2±11.4)×10~9/L,对照组小鼠治疗后PLT数值为(375.2±12.6)×10~9/L,观察组小鼠治疗后PLT数值明显高于对照组小鼠,有显著差异,具有统计学意义(P<0.05)。观察组患者治疗后骨髓有核细胞数(8.6±1.5)×10~9/L,对照组患者治疗后骨髓有核细胞数(17.9±1.8)×10~9/L,观察组明显高于对照组,二者差异明显,具有统计学意义(P<0.05)。观察组患者治疗后脾脏指数为(9.7±1.3)mg/g,对照组患者为(4.1±1.9)mg/g,观察组明显高于对照组,二者差异明显,具有统计学意义(P<0.05)。观察组小鼠各T细胞分泌细胞因子IFN-r分泌量为(17.9±1.8)pg/mL;TNF-a分泌量为(32.5±3.5)pg/mL;IL-4分泌量为(5.59±0.89)pg/mL;IL-6分泌量为(16.1±1.9)pg/mL。对照组小鼠IFN-r分泌量为(7.6±1.5)pg/mL;TNF-a分泌量为(21.5±2.5)pg/mL;IL-4分泌量为(4.48±0.23)pg/mL;IL-6分泌量为(7.7±1.3)pg/mL,观察组小鼠T细胞分泌的细胞因子各项指标均高于对照组小鼠,二者差异明显,具有统计学意义(P<0.05)。结论:茯贞膏能够显著改善贫血小鼠血象,对提高小鼠造血功能有积极影响,可试行二期临床。

Objective：To investigate the effect of Fuzhen Ointment on anemia of hematopoiesis in mice.Methods：A total of 128 BALB/c mice were selected as the research object.First adaptively feed for 1 weekand then use the physical radiation method toset up the cyclophosphamide-induced anemia model.At the end of the project,128 BALB/c mice were in accordance with the random number table method divided into the observation group and the control group.The observation group had 64 mice and control group 64 mice.Two groups’ mice used different administration ways.Give the observation group Fuzhen Ointmentdaily,9g/kg,9 d for a cycle,continuous for a cycle.The control group was given normal saline by gavage and the dose was the same as that of the observation group,for 9 days.After 9 days of treatment,two groups’ bone marrow nuclear cell counts and spleen indexwere compared and analyzed.Results：After treatment,the observation group’s RBC value was（9.6±1.5）×1012/L and the control group’s only（7.9±1.8）×1012/L.The observation group’s RBC numerical value was significantly higher than that of the control group and the difference was statistically significant（P〈0.05）.The observation group’s WBC value of mice after treatment was（19.7±1.3）×109/L and the control group’s value of WBC was（6.1±1.9）×1012/L.The control group’s was significantly higher than that ofthe observation groupwith statistical significance（P〈0.05）.The observation group’s HB value after treatment was（199.5±5.6） g/L,significantly higher than the control group’s（159.1±4.8） g/L.The differences had statistical significance（P〈0.05）.The observation group’s PLT valueof mice after treatment was（488.2±11.4）×109/L and the control group’s（375.2±12.6）×109/L.The observation group’s PLT valueof mice after treatment was significantly higher than the control group’s.There were significant differences（P〈0.05）.The patients’ bone marrow nucleated cell count in the observation group were（8.6±1.5）×109/L and the control group’s（17.9±1.8）×109/L.The observation group’s was significantly higher than that in the control group and there were significant differences（P〈0.05）.The spleen index in the observation group was（9.7±1.3） mg/g and the control group was（4.1±1.9） mg/g.The observation group’s was significantly higher than that of the control group and the difference between the two groups was significant（P〈0.05）.Observation group’s cytokines IFN-r secreted by T cell was（17.9±1.8） pg/mL;TNF-a secretion（32.5±3.5） pg/mL;IL-4 secretion（5.59±0.89） pg/mL;IL-6 secretion for（16.1±1.9） pg/mL.Control group’s IFN-r secretion was（7.6±1.5） pg/mL;TNF-a secretion（21.5±2.5） pg/mL;IL-4 secretion（4.48±0.23） pg/mL;IL-6 secretion（7.7±1.3） pg/mL.The cytokines indicators in the observation group were higher than those of the control group with statistical significance（P〈0.05）.Conclusion：Fuzhen Ointment can significantly improve anemia in mice and has a positive impact on the hematopoietic function and can carry out the second stage clinical trial.