利用产气荚膜梭菌作为泳滩水质污染指标菌的检测方法

Use of Clostridium perfringens as fecal indicator of bathing water

目的评估产气荚膜梭菌作为泳滩水质污染指标菌,建立更快速及可靠的方法。方法抽取澳门黑沙海滩及竹湾海滩的泳滩水作为样本,比较双套管法定量检测产气荚膜梭菌,利用滤膜法定量检测大肠埃希菌数。结果产气荚膜梭菌及大肠埃希菌结果数据无相关性(P>0.05)。粪大肠菌群与大肠埃希菌呈正相关(P<0.05)。厌氧亚硫酸盐还原梭菌与产气荚膜梭菌呈正相关(P<0.05)。产气荚膜梭菌采样点平均值不合格数远多于大肠埃希菌不合格数(P<0.05)。结论利用双套管法定量检测产气荚膜梭菌方便快捷,符合公共卫生检测上的效益,更全面反映水质污染情况,故此建议加入产气荚膜梭菌作为澳门泳滩水的指标微生物。

Objective To evaluate the use of Clostridium perfringens as the indicator of beach water quality,in order to establish a faster and reliable method. Methods Beach water samples from Hac-Sa Beach and Coloane Beach were collected in this study. Enumeration of Clostridium perfringens by the Fung Double Tube method（ FDT） and Escherichia coli by membrane filtration method were studied. Results Comparing the result and find that there was no correlation between Clostridium perfringens and Escherichia coli（ P〈0. 05）. However,fecal coliforms and E. coli showed poisitive correlation（ P〈0. 05）; and sulfite reducing clostridia and Clostridium perfringens showed positive correlation too（ P〈0. 05）. The average substandard count of Clostridium perfringens sampling site was far excess than that of the Escherichia coli sampling site（ P〈0. 05）. Conclusion Using FDT to detect Clostridium perfringens quantitatively is faster and reliable,which can comply with the benefits of public health detection and can more fully reflect the water pollution situation,so Clostridium perfringens is proposed to become a member of Macao beach water＇s indicator.