盐酸恩诺沙星对蛋白核小球藻(Chlorella pyrenoidosa)的毒性试验

Toxic Effects of Enrofloxacin hydrochloride on Chlorella pyrenoidosa

为探讨水产养殖中氟喹诺酮类药物残留对水生生物的毒性效应,本试验研究了盐酸恩诺沙星对蛋白核小球藻(Chlorella pyrenoidosa)的毒性作用。试验设置5个药物浓度组和1个试验对照组。结果显示,各药物浓度组对蛋白核小球藻的生长均有抑制作用,并且随药物处理浓度的增大,藻细胞的生长逐渐降低。叶绿素a、b含量随着盐酸恩诺沙星处理浓度的增大而下降。蛋白核小球藻在经过不同浓度的恩诺沙星处理96 h后,各组蛋白质含量均远远低于对照组,并且蛋白质含量随着恩诺沙星浓度的升高逐渐减少。超氧化物歧化酶(SOD)活力和膜脂过氧化产物MDA则随着恩诺沙星药品浓度的升高而升高。表明盐酸恩诺沙星对蛋白核小球藻的胁迫引起其可溶性蛋白质含量、超氧化物歧化酶(SOD)活力和膜脂过氧化产物MDA含量的变化。

In order to study the toxic effects of fluorequinolones on the fresh algae, Chlorella pyrenoidosa was exposed to Enrofloxacin hydroehloride of five concentrations （ 15. 625, 31.25, 50, 62.5, 125 mg· L- 1 ） and a control. Results showed that, Enrofloxaein hydrochloride had inhibition effect on the growth of Chlorella pyrenoidosa compared with the control. With the increase of concentrations, the growth of algae cells gradually decreased correspondingly. Enrofloxacin hydrochloride had the same effects on the contents of the chlorophyll-a, chlorophyU-b, that is, the contents of the photosynthetic pigments decreased. The activity of Superexide dismutase （SOD） and Malondialdehyde （ MDA ） were increased and protein content reduced with increased concentration of Enrofloxaein.