艾炷灸对衰老小鼠学习记忆力及胰岛素水平的影响

Effect of moxa cone moxibustion on learning and memory abilities and insulin level in senescent mice

目的:观察艾炷灸对D-半乳糖衰老小鼠学习记忆及血清、脑组织胰岛素(INS)水平的影响。方法:将75只小鼠随机分为对照组、造模组、艾灸1组、艾灸2组、艾灸3组,每组15只。艾灸1组艾炷灸足三里穴+悬钟穴,艾灸2组艾炷灸百会穴+关元穴,艾灸3组艾炷灸腕背关节上1mm与膝髌正中上沿上2mm。用Morris水迷宫测试学习记忆力,用酶联免疫法检测血清及脑组织INS水平。结果:治疗前各组小鼠水迷宫各项指标及学习记忆能力比较,差异无统计学意义(P>0.05);治疗后,造模组与对照组、艾灸1组、艾灸2组比较,各项指标差异均有统计学意义(P<0.01或P<0.05);对照组、艾灸1组、艾灸2组与艾灸3组比较,差异均有统计学意义(P<0.05)。造模组血清和脑组织INS水平较对照组、艾灸1组和艾灸2组升高或降低,差异均有统计学意义(P<0.01或P<0.05);对照组、艾灸1组、艾灸2组与艾灸3组比较,差异均有统计学意义(P<0.05)。结论:艾炷灸足三里穴+悬钟穴、关元穴+百会穴均能显著提高D-半乳糖衰老小鼠学习记忆能力,与降低外周循环胰岛素水平、提高脑组织的胰岛素浓度有关,具有延缓脑衰老作用。

Objective： To investigate the effect of moxa cone moxibustion on learning and memory abilities and insulin（ INS） level in serum and cerebral tissue in mice with senescence induced by D-galactose. Methods： A total of 75 mice were randomly divided into control group,model group,and moxibustion groups 1,2,and 3,with 15 mice in each group. The mice in moxibustion group 1 were given moxa cone moxibustion at Zusanli and Xuanzhong points,those in moxibustion group 2 were given moxa cone moxibustion at Baihui and Guanyuan points,and those in moxibustion group 3 were given moxa cone moxibustion at 1mm above the waist back joint and 2mm above the upper edge of the middle knee-patellar joint. The Morris water maze was used to test learning and memory abilities,and ELISA was used to measure INS level in serum and cerebral tissue. Results： There were no significant differences in the indices of water maze test and learning and memory abilities between groups before treatment（ P〉0. 05）; after treatment,there were significant differences in these indices between the model group and the control group/moxibustion groups 1 and 2（ P〈0. 01 or P〈0. 05）,as well as between the control group/moxibustion groups 1 and 2 and moxibustion group 3（ P〈0. 05）. Compared with the control group and moxibustion groups 1 and 2,the model group had a significant increase or reduction in the INS level in serum and cerebral tissue（ P〈0. 01 or P〈0. 05）,and there was a significant difference between the control group/moxibustion groups 1 and 2 and moxibustion group 3（ P〈0. 05）. Conclusion： Moxa cone moxibustion at Zusanli and Xuanzhong points or Baihui and Guanyuan points can significantly improve the learning and memory abilities of mice with senescence induced by D-galactose,possibly by reducing INS level in peripheral circulation and increasing INS level in cerebral tissue,and therefore,it can delay brain aging.